¿Qué tipos de pruebas rápidas para la COVID-19 se han desarrollado?

(What types of rapid testing for covid-19 have been developed?)

Primeras 5 respuestas:

Immunoenzymatic Assay (ELISA), Chemiluminescence, Electro Chemiluminescence, Fluorescent Lateral Flow Immunoassays and Immunochromatography.

If multiple blood samples were collected from a patient with COVID-19 on the same day, only one sample was used for antibody rapid testing.

We created three working groups that focused on implementing manual RT-PCR testing, automated/commercial PCR testing and serologic testing.

The IgM-IgG combined assay has better utility and sensitivity compared with a single IgM or IgG test.

Different diagnostic test manufacturers have developed rapid tests based on SARS-CoV-2 proteins detection in respiratory samples.

Immunoenzymatic Assay (ELISA), Chemiluminescence, Electro Chemiluminescence, Fluorescent Lateral Flow Immunoassays and Immunochromatography.

... Background The COVID-19 serological tests for IgG and IgM have been developed with several methodologies: Immunoenzymatic Assay (ELISA), Chemiluminescence, Electro Chemiluminescence, Fluorescent Lateral Flow Immunoassays and Immunochromatography. None of these tests should be used for the diagnosis or population screening of the ...

Ref: Concordance of "rapid" serological tests and IgG and IgM chemiluminescence for SARS-COV-2 [bioRxiv, 2020-06-03]

If multiple blood samples were collected from a patient with COVID-19 on the same day, only one sample was used for antibody rapid testing.

... attending physicians providing regular medical care. All blood samples were collected on the date of venipuncture. If multiple blood samples were collected from a patient with COVID-19 on the same day, only one sample was used for antibody rapid testing. The serum samples collected from the study patients were stored at -20ºC before use. ...

Ref: Four point-of-care lateral flow immunoassays for diagnosis of COVID-19 and for assessing dynamics of antibody responses to SARS-CoV-2 [J Infect, 2020-06-15]

We created three working groups that focused on implementing manual RT-PCR testing, automated/commercial PCR testing and serologic testing.

... reference laboratories, but to meet our needs, we needed to rapidly develop in-house COVID-19 testing. We created three working groups that focused on implementing manual RT-PCR testing, automated/commercial PCR testing and serologic testing. Each group was charged with developing diversified testing platforms, in order to achieve the flexibility ...

Ref: A how-to-guide to building a robust SARS-CoV-2 testing program at a university-based health system [J Infect, 2020-06-08]

The IgM-IgG combined assay has better utility and sensitivity compared with a single IgM or IgG test.

... indicated great detection consistency among samples from fingerstick blood, serum and plasma of venous blood. The IgM-IgG combined assay has better utility and sensitivity compared with a single IgM or IgG test. It can be used for the rapid screening of SARS-CoV-2 carriers, symptomatic or asymptomatic, in ...

Ref: Development and clinical application of a rapid IgM-IgG combined antibody test for SARS-CoV-2 infection diagnosis [J. med. virol, 2020]

Different diagnostic test manufacturers have developed rapid tests based on SARS-CoV-2 proteins detection in respiratory samples.

... Different diagnostic test manufacturers have developed rapid tests based on SARS-CoV-2 proteins detection in respiratory samples. However, the analytical performances of these rapid antigenic tests depend on different factors including the viral load, the quality of the specimen and how it is processed. The performances also ...

Ref: Low performance of rapid antigen detection test as frontline testing for COVID-19 diagnosis [J Clin Virol, 2020-05-21]

Numerous serologic immunoassays have been launched to detect antibodies to SARS-CoV-2, including rapid tests.

... Objectives: Numerous serologic immunoassays have been launched to detect antibodies to SARS-CoV-2, including rapid tests. Here, we validate use of a lateral flow immunoassay (LFI) intended for rapid screening and qualitative detection of anti-SARS-CoV-2 IgM and IgG in serum, plasma, and whole blood, and ...

Ref: Analytical performance of lateral flow immunoassay for SARS-CoV-2 exposure screening on venous and capillary blood samples [J Clin Virol, 2020-05-18]

nucleic acid real-time polymerase chain reaction (PCR) test has become the standard method for diagnosis of SARS-CoV-2 infection,

... to more than 20 other countries. Although the virus (severe acute respiratory syndrome coronavirus [SARS-Cov-2]) nucleic acid real-time polymerase chain reaction (PCR) test has become the standard method for diagnosis of SARS-CoV-2 infection, these real-time PCR test kits have many limitations. In addition, high false-negative rates were reported. ...

Ref: Development and clinical application of a rapid IgM-IgG combined antibody test for SARS-CoV-2 infection diagnosis [J. med. virol, 2020]

The RT-PCR test for identificationof viral nucleic acid is the current standard diagnostic method for the diagnosis of COVID-19.

... The RT-PCR test for identificationof viral nucleic acid is the current standard diagnostic method for the diagnosis of COVID-19. However, this assay has some practical limitations (3)such as the annoying method to obtain biological material from the nasopharynx, the relatively long time to generate results, the need for certified ...

Ref: Clinical meanings of rapid serological assay in patients tested for SARS-Co2 RT-PCR [J. med. virol, 2020-04-06]

Nucleic acid testing for viral pathogen and serological testing for host antibodies are playing important roles in current COVID-19 diagnosis.

... has now evolved into a global pandemic. Early detection is crucial for its effective control. Nucleic acid testing for viral pathogen and serological testing for host antibodies are playing important roles in current COVID-19 diagnosis. However, while nucleic acid testing is complicated, facility-restricted and time-consuming, antibody testing may result in ...

Ref: Ultra-sensitive nanozyme-based chemiluminescence paper test for rapid diagnosis of SARS-CoV-2 infection [bioRxiv, 2020-06-05]

The rapid LFIA and automated CLIA tests for IgM and IgG could complement the existing COVID-19 testing by RT-PCR.

... passed the disease onto many others is contributing to the continued global spread of COVID-19. The rapid LFIA and automated CLIA tests for IgM and IgG could complement the existing COVID-19 testing by RT-PCR. However, there is a need to stringently evaluate the clinical performance of commercial tests before ...

Ref: In Vitro Diagnostic Assays for COVID-19: Recent Advances and Emerging Trends [Diagnostics (Basel), 2020-04-05]

Many nucleic acid tests based on reverse transcriptase-polymerase chain reaction (RT-PCR) were developed,

... allow clinical and commercial laboratories to develop tests to detect the presence of the virus. Many nucleic acid tests based on reverse transcriptase-polymerase chain reaction (RT-PCR) were developed, each with different techniques, specifications and turnaround time. As the illnesses turned into a pandemic, ...

Ref: Clinical Testing For Covid-19 [J Allergy Clin Immunol, 2020-05-20]

We tested two promising candidate lateral flow rapid fingerprick test with bands for IgG and IgM.

... ELISA test to detect IgG antibodies to nucleocapsid (N) and spike (S) proteins from SARS-CoV-2. We tested two promising candidate lateral flow rapid fingerprick test with bands for IgG and IgM. We then prospectively recruited participants with suspected moderate to severe COVID-19 and tested for SARS-CoV-2 ...

Ref: Combined point of care nucleic acid and antibody testing for SARS-CoV-2: a prospective cohort study in suspected moderate to severe COVID-19 disease. [J Allergy Clin Immunol, 2020-06-18]

A serologic test to identify antibody dynamics and response to SARS-CoV-2 was developed.

... (COVID-19) caused by the corona virus 2 (SARS-CoV-2) has been rapidly spreading nationwide and abroad. A serologic test to identify antibody dynamics and response to SARS-CoV-2 was developed. METHODS: The antibodies against SARS-CoV-2 were detected by an enzyme-linked immunosorbent assay (ELISA) based on ...

Ref: Antibody Detection and Dynamic Characteristics in Patients with COVID-19 [Clin Infect Dis, 2020-04-19]

The routinely used realtime RT-PCR is sensitive, specific and able to process large batches of samples.

... BACKGROUND: With the outbreak of SARS-CoV-2, rapid diagnostics are paramount to contain the current pandemic. The routinely used realtime RT-PCR is sensitive, specific and able to process large batches of samples. However, turnaround time is long and in cases where fast obtained results are critical, molecular ...

Ref: Multi-center evaluation of cepheid xpert® xpress SARS-CoV-2 point-of-care test during the SARS-CoV-2 pandemic [J Clin Virol, 2020]

More than 200 different assays have been proposed so far but almost all have poor regulatory status and lack clinical and analytical performance review.

... the need to accelerate progress in diagnostics, serological tests have been developed. More than 200 different assays have been proposed so far but almost all have poor regulatory status and lack clinical and analytical performance review. 8 In fact the speed with which they are released in the market and the versatility of immunoassays ...

Ref: Diagnostic accuracy of an automated chemiluminescent immunoassay for anti‐SARS‐CoV‐2 IgM and IgG antibodies: an Italian experience [J Med Virol, 2020-05-10]

Real-time reverse transcriptase polymerase chain reaction–based assays performed in a laboratory on respiratory specimens are the reference standard for COVID-19 diagnostics.

... United States, hampered by limited testing capacity, has prioritized testing for specific groups of persons. Real-time reverse transcriptase polymerase chain reaction–based assays performed in a laboratory on respiratory specimens are the reference standard for COVID-19 diagnostics. However, point-of-care technologies and serologic immunoassays are rapidly emerging. Although excellent tools exist for the ...

Ref: Diagnostic Testing for Severe Acute Respiratory Syndrome–Related Coronavirus-2: A Narrative Review [Ann Intern Med, 2020-04-13]

Between 16, March and 14, April, 2020, we performed rapid IgM and IgG detecting tests without PCR;

... of real-time PCR tests considered as the gold standard in the detection of infection. Method: Between 16, March and 14, April, 2020, we performed rapid IgM and IgG detecting tests without PCR; PCR without rapid tests; and PCR WITH rapid tests in 4140, 3210 and 1654 patients, ...

Ref: [A specifikus IgM- és IgG-antitesteket detektáló gyorstesztek értéke a SARS CoV-2 vírusfertozés kimutatásában (A COVID-19-pandémia orvosszakmai kérdései)]/ The diagnostic value of rapid anti IgM and IgG detecting tests in the identification of patients with SARS CoV-2 virus infection [Orv Hetil, 2020]

Wantai SARS-COV-2 Ab ELISA and Orient Gene COVID-19 IgG/IgM Rapid Test achieved a superior overall sensitivity.

... 0 to 54 days after symptom onset. Specificity was verified on 57 pre-pandemic samples. Results Wantai SARS-COV-2 Ab ELISA and Orient Gene COVID-19 IgG/IgM Rapid Test achieved a superior overall sensitivity. Elecsys Anti-SARS-CoV-2 assay, EUROIMMUN Anti-SARS-CoV-2 combined IgG/IgA and EUROIMMUN Anti-SARS-CoV-2-NCP(IgG) also showed acceptable sensitivity (>95%) ...

Ref: Kinetics of the humoral immune response to SARS-CoV-2: comparative analytical performance of seven commercial serology tests [Orv Hetil, 2020-06-12]

The authors use a specific real-time polymerase chain reaction test (RT-PCR) as standard laboratory reference method.

... anti-SARS-CoV-2 virus antibodies (IgG/IgM) is evaluated for sensitivity and specificity to detect the viral infection. The authors use a specific real-time polymerase chain reaction test (RT-PCR) as standard laboratory reference method. They found that the antibody rapid test only detects 36.4 % of the samples identified ...

Ref: Comment to Döhla et al., Rapid point-of-care testing for SARS-CoV- 2 in a community screening setting shows low sensitivity [Public Health, 2020-06-02]

We evaluated a lateral flow immunoassay (AllTest COVID-19 IgG/IgM) which detects IgG and IgM antibodies.

... was to analyze the diagnostic performance of one serologic rapid test in COVID-19 patients. METHODS: We evaluated a lateral flow immunoassay (AllTest COVID-19 IgG/IgM) which detects IgG and IgM antibodies. We validated the serologic test using serum samples from 100 negative patients (group 1) and ...

Ref: Alltest rapid lateral flow immunoassays is reliable in diagnosing SARS-CoV-2 infection from 14 days after symptom onset: A prospective single-center study [J Clin Virol, 2020]

A multi-tiered, diagnostic strategy incorporating a rapid host immune response assay as a screening test, molecular confirmatory testing and rapid IgM/IgG testing

... the ability to efficiently screen patients, hospitals are overwhelmed, potentially delaying treatment for other emergencies. A multi-tiered, diagnostic strategy incorporating a rapid host immune response assay as a screening test, molecular confirmatory testing and rapid IgM/IgG testing to assess benefit from quarantine/further testing and provide information on population exposure/herd immunity would efficiently ...

Ref: Multi-tiered screening and diagnosis strategy for COVID-19: a model for sustainable testing capacity in response to pandemic [Ann Med, 2020]

Serology testing allows for the detection of asymptomatic infections and 19-COVID cases once the virus has been cleared.

... reasons, to allow the best treatment of cases and the best control of the pandemic. Serology testing allows for the detection of asymptomatic infections and 19-COVID cases once the virus has been cleared. We analyzed the usefulness of the SARS-CoV-2 rapid test of Autobio and tried to correlate ...

Ref: Utility of lateral flow tests in SARS-CoV-2 infection monitorization. [Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia, 2020-06-04]

In order to overcome these limitations, different microplate ELISA tests have been developed.

... In order to overcome these limitations, different microplate ELISA tests have been developed. Recently published studies confirm the usefulness of combining PCR in nasopharyngeal exudates and the detection of IgM and IgG antibodies in the blood of patients (8). The combination of both ...

Ref: Rapid diagnosis of SARS-CoV-2 infection by detecting IgG and IgM antibodies with an immunochromatographic device: a prospective single-center study [Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia, 2020-04-15]

The study was conducted performing Prima Lab SA Covid-19 IgG/IgM Rapid Test,

... The mean age was 42.9 years old, while the median age was 38 years old. The study was conducted performing Prima Lab SA Covid-19 IgG/IgM Rapid Test, a qualitative membrane-based immunoassay for the detection of IgG and IgM antibodies to Covid-19 in ...

Ref: Evaluating the adequacy of Prima Covid-19 IgG/IgM Rapid Test for the assessment of exposure to SARS-CoV-2 virus [Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia, 2020-06-03]

Currently, screening for COVID-19 is performed by checking for high fever in individuals coming through airports and border controls using thermal screening guns.

... procedures, including strict security screening for passengers and baggage at all commercial airports 41 . Currently, screening for COVID-19 is performed by checking for high fever in individuals coming through airports and border controls using thermal screening guns. A nanosensor diagnostic platform is in development to test for this virus, which will replace ...

Ref: Current and emerging diagnostic tests available for the novel COVID-19 global pandemic [AAS Open Res, 2020-04-24]

Rapid Diagnostic Tests (RDTs) have been developed to detect specific antibodies, IgG and IgM,

... the infectious respiratory disease called COVID-19 (COronaVIrus Disease). In response to the growing COVID-19 pandemic, Rapid Diagnostic Tests (RDTs) have been developed to detect specific antibodies, IgG and IgM, to SARS-CoV-2 virus in human whole blood. We conducted a real-life study to evaluate the ...

Ref: Evaluation of performance of two SARS-CoV-2 Rapid whole-blood finger-stick IgM-IgGCombined Antibody Tests [AAS Open Res, 2020-05-27]

Recently, several easy to perform rapid antigen detection tests were developed and recommended in some countries as the first line of diagnostic.

... requirement of special equipment and skilled staff limit the use of these time-consuming molecular techniques. Recently, several easy to perform rapid antigen detection tests were developed and recommended in some countries as the first line of diagnostic. OBJECTIVES: The aim of this study was to evaluate the performances of the Coris COVID-19 ...

Ref: Low performance of rapid antigen detection test as frontline testing for COVID-19 diagnosis [J Clin Virol, 2020]

Nucleic acid testing is the standard method for the diagnosis of viral infections.

... (SARS‐CoV‐2), has rapidly developed into a pandemic since it was first reported in December 2019. Nucleic acid testing is the standard method for the diagnosis of viral infections. However, this method reportedly has a low positivity rate. To increase the sensitivity of COVID‐19 ...

Ref: Characteristics of patients with coronavirus disease (COVID‐19) confirmed using an IgM‐IgG antibody test [J Med Virol, 2020-05-07]

Real-time reverse transcriptase polymerase chain reaction-based assays performed in a laboratory on respiratory specimens are the reference standard for COVID-19 diagnostics.

... United States, hampered by limited testing capacity, has prioritized testing for specific groups of persons. Real-time reverse transcriptase polymerase chain reaction-based assays performed in a laboratory on respiratory specimens are the reference standard for COVID-19 diagnostics. However, point-of-care technologies and serologic immunoassays are rapidly emerging. Although excellent tools exist for the ...

Ref: Diagnostic Testing for Severe Acute Respiratory Syndrome-Related Coronavirus 2: A Narrative Review [Ann Intern Med, 2020]

Serologic assays have been developed to detect infection with coronavirus disease 2019 (COVID-19).

... Serologic assays have been developed to detect infection with coronavirus disease 2019 (COVID-19). This study was conducted to evaluate the diagnostic performance of an immunochromatography-based assay of human serum for COVID-19. The present study enrolled 149 subjects who had been tested by real-time ...

Ref: Diagnostic performance of immunochromatography assay for rapid detection of IgM and IgG in coronavirus disease 2019 [J. med. virol, 2020]

Chest computed tomography (CT) is a valuable component in the evaluation of patients with suspected SARS-CoV-2 infection.

... the urgent need for alternative methods for rapid and accurate diagnosis of patients with COVID-19. Chest computed tomography (CT) is a valuable component in the evaluation of patients with suspected SARS-CoV-2 infection. Nevertheless, CT alone may have limited negative predictive value for ruling out SARS-CoV-2 infection, as ...

Ref: Artificial intelligence-enabled rapid diagnosis of patients with COVID-19. [Nature medicine, 2020-05-19]

Thirty-nine randomly selected individuals at a COVID-19 screening centre were simultaneously tested via qPCR and a rapid test.

... too long. We therefore evaluated a rapid test in a high-prevalence community setting. STUDY DESIGN: Thirty-nine randomly selected individuals at a COVID-19 screening centre were simultaneously tested via qPCR and a rapid test. Ten previously diagnosed individuals with known SARS-CoV-2 infection were also analysed. METHODS: The evaluated rapid ...

Ref: Rapid point-of-care testing for SARS-CoV-2 in a community screening setting shows low sensitivity [Public Health, 2020]

The purpose of this study was to assess the performance of five immunoassays for the detection of SARS-CoV-2 antibodies.

... INTRODUCTION: Several SARS-CoV-2 immunoassays have been developed recently. The purpose of this study was to assess the performance of five immunoassays for the detection of SARS-CoV-2 antibodies. METHODS: Two quantitative automated immunoassays (Maglumi™2019-n-Cov IgG and IgM and Euroimmun Anti-SARS-CoV-2 IgG and IgA assays) and three lateral flow rapid tests ...

Ref: Evaluation of two automated and three rapid lateral flow immunoassays for the detection of anti-SARS-CoV-2 antibodies [J Clin Virol, 2020]

Serological tests based on point-of-care (POC) lateral flow immunoassays (LFIAs)

... Serological tests based on point-of-care (POC) lateral flow immunoassays (LFIAs) have been developed to detect anti-SARS-CoV-2 antibodies simultaneously or separately for the diagnosis of COVID-19. [8] [9] [10] Compared to rRT-PCR, serological tests have the advantage of decreased technical requirement, ...

Ref: Four point-of-care lateral flow immunoassays for diagnosis of COVID-19 and for assessing dynamics of antibody responses to SARS-CoV-2 [J Infect, 2020-06-15]

The rapid test used for evaluation is a qualitative IgG/IgM detection system to test for a current or past infection of SARS-CoV-2.

... The rapid test used for evaluation is a qualitative IgG/IgM detection system to test for a current or past infection of SARS-CoV-2. The chemical coupling pad contains gold-labelled SARS-CoV-2 Weak and strong is superior; the manufacturer"s recommendation is also to interpret weak results as positive. LRþ: positive likelihood ratio; LR-: negative likelihood ...

Ref: Rapid point-of-care testing for SARS-CoV-2 in a community screening setting shows low sensitivity [Public Health, 2020-04-18]

Diagnosis of suspected cases is confirmed by nucleic acid assays with real-time PCR, using respiratory samples.

... World Health Organization (WHO) declared COVID-19 outbreak as a world pandemic on 12th March 2020. Diagnosis of suspected cases is confirmed by nucleic acid assays with real-time PCR, using respiratory samples. Serology tests are comparatively easier to perform, but their utility may be limited by the ...

Ref: Diagnostic performance of COVID-19 serology assays. [The Malaysian journal of pathology, 2020-04-01]

Rapid and accurate tests that detect IgM and IgG antibodies to SARS-CoV-2 proteins are essential in slowing the spread of COVID-19

... Rapid and accurate tests that detect IgM and IgG antibodies to SARS-CoV-2 proteins are essential in slowing the spread of COVID-19 by identifying patients who are infected with COVID-19. Using a SARS-CoV-2 proteome microarray developed in our lab, we comprehensively profiled both IgM and IgG antibodies in forty patients with early-stage ...

Ref: Proteome-wide analysis of differentially-expressed SARS-CoV-2 antibodies in early COVID-19 infection [The Malaysian journal of pathology, 2020-04-20]

Serologic assays have been developed to detect infection with coronavirus disease 2019 (COVID‐19).

... Serologic assays have been developed to detect infection with coronavirus disease 2019 (COVID‐19). This study was conducted to evaluate the diagnostic performance of an immunochromatography‐based assay of human serum for COVID‐19. The present study enrolled 149 subjects who had been tested by real‐time ...

Ref: Diagnostic performance of immunochromatography assay for rapid detection of IgM and IgG in coronavirus disease 2019 [J Med Virol, 2020-06-09]

LDT RT-qPCR, cobas SARS-CoV-2 high-throughput system, direct RT-qPCR except for one commercial kit,

... in saliva from males than females. Conclusions Self-collected saliva is an alternative specimen diagnosing COVID-19. LDT RT-qPCR, cobas SARS-CoV-2 high-throughput system, direct RT-qPCR except for one commercial kit, and RT-LAMP showed sufficient sensitivity in clinical use to be selectively used according to clinical ...

Ref: Clinical evaluation of self-collected saliva by RT-qPCR, direct RT-qPCR, RT-LAMP, and a rapid antigen test to diagnose COVID-19 [J Med Virol, 2020-06-08]

Imaging technologies, such as chest CT scans and ultrasound, have been proposed as tools to diagnose COVID-19.

... Imaging technologies, such as chest CT scans and ultrasound, have been proposed as tools to diagnose COVID-19. Chest CT scans can identify cases of viral pneumonia, even though RT-PCR results for SARS-CoV-2 at the time images are taken are negative [7] . A retrospective study of 1,014 ...

Ref: What Do We Need to Know to Improve Diagnostic Testing Methods for the 2019 Novel Coronavirus? [Cureus, 2020-05-24]

Laboratory confirmation of COVID-19 has been based on a positive real-time RT-PCR result.

... diversity of symptoms and imaging findings, molecular and serological detection tools are rapidly being developed. Laboratory confirmation of COVID-19 has been based on a positive real-time RT-PCR result. However, molecular and serological studies on this virus appear to be scarce. In this study, ...

Ref: Diagnostic value and dynamic variance of serum antibody in coronavirus disease 2019 [International Journal of Infectious Diseases, 2020-05-31]

Between 16, March and 14, April, 2020, we performed rapid IgM and IgG detecting tests without PCR; PCR without rapid tests;

For confirmed COVID-19 cases, reported common clinical symptoms include fever, cough, myalgia, or fatigue.

... are limited by one hard problem: how to differentiate the COVID-19 cases from the healthy. For confirmed COVID-19 cases, reported common clinical symptoms include fever, cough, myalgia, or fatigue. 8 Yet these symptoms are not unique features of COVID-19 because these symptoms are similar ...

Ref: Development and clinical application of a rapid IgM‐IgG combined antibody test for SARS‐CoV‐2 infection diagnosis [J Med Virol, 2020-04-13]

SARS-CoV-2-associatedimmunoglobulinswere assayed by utilizing a rapid serological colorimetric test.

... insight into this aspect of the epidemic, we tested525 asymptomatic workers of our Cancer Institute where immuno-depressed patients are extremely frequent and sporadic COVID-19 positive cases had recently been reported. SARS-CoV-2-associatedimmunoglobulinswere assayed by utilizing a rapid serological colorimetric test. ...

Ref: RAPID SEROLOGICAL TESTS HAVE A ROLE IN ASYMPTOMATIC HEALTH WORKERS COVID-19 SCREENING [J Med Virol, 2020-04-17]

Timely and accurate COVID-19 laboratory testing is an essential step in the management of the COVID-19 outbreak.

... SARS-CoV-2 was discovered in late December 2019 and, since then, has become a global pandemic. Timely and accurate COVID-19 laboratory testing is an essential step in the management of the COVID-19 outbreak. To date, assays based on the reverse-transcription polymerase chain reaction (RT-PCR) in respiratory samples are ...

Ref: Point-of-Care Diagnostic Tests for Detecting SARS-CoV-2 Antibodies: A Systematic Review and Meta-Analysis of Real-World Data [J Med Virol, 2020]

several serological tests, including the enzyme-linked immunosorbent assay (ELISA), the chemiluminescence immunoassay (CLIA) and lateral flow rapid testing,

... for research on in-vitro diagnostics for use at the community level [4] . In response, several serological tests, including the enzyme-linked immunosorbent assay (ELISA), the chemiluminescence immunoassay (CLIA) and lateral flow rapid testing, are now under development or have already entered the market. The Foundation for Innovative New ...

Ref: Evaluation of two automated and three rapid lateral flow immunoassays for the detection of anti-SARS-CoV-2 antibodies [J Clin Virol, 2020-05-05]

rapid lateral flow assays for antibodies (IgM and IgG) produced during COVID-19 have been developed (70) .

... As mentioned above, rapid lateral flow assays for antibodies (IgM and IgG) produced during COVID-19 have been developed (70) . Seroconversion occurred after 7 days of symptomatic infection in 50% of patients (14 days in all) but was not followed by a rapid decline in viral load ...

Ref: Laboratory Diagnosis of COVID-19: Current Issues and Challenges [J Clin Microbiol, 2020-05-26]

Rapid antigen detection (RAD) tests are available, however, there is scanty data on the performance of RAD tests.

... BACKGROUND: The rapid diagnosis of COVID-19 patients is essential to reduce the disease spread. Rapid antigen detection (RAD) tests are available, however, there is scanty data on the performance of RAD tests. OBJECTIVE: To evaluate the performance of BIOCREDIT COVID-19 Ag test and compared it with real-time RT-PCR ...

Ref: Evaluation of rapid antigen test for detection of SARS-CoV-2 virus [J Clin Virol, 2020-06-08]

185 (5.3%) employees tested positive for COVID-19.

... a total of 3,477 symptomatic employees were tested for COVID-19 at two employee testing centers; 185 (5.3%) employees tested positive for COVID-19. The prevalence of SARS-CoV-2 was similar when comparing frontline HCWs (5.2%) to non-frontline staff (5.5%). ...

Ref: Prevalence of COVID-19 Infection and Outcomes Among Symptomatic Healthcare Workers in Seattle, Washington. [Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, 2020-06-16]

So far, there is limited data on how commercially available tests perform with real patient samples and if positive tested samples show neutralizing abilities.

... SARS-CoV-2 serological assays are urgently needed for rapid diagnosis, contact tracing and for epidemiological studies. So far, there is limited data on how commercially available tests perform with real patient samples and if positive tested samples show neutralizing abilities. Focusing on IgG antibodies, we demonstrate the performance of two ELISA assays (Euroimmun SARS-CoV-2 IgG ...

Ref: Clinical performance of different SARS-CoV-2 IgG antibody tests. [Journal of medical virology, 2020-06-08]

The samples were tested using RT-LAMP as well as by conventional qRT-PCR.

... nucleic sequence. RNA isolated from nasopharyngeal swabs collected from actual COVID-19 patients was also tested. The samples were tested using RT-LAMP as well as by conventional qRT-PCR. Specificity of the RT-LAMP was evaluated by also testing against other related coronaviruses. RT-LAMP specifically ...

Ref: Rapid detection of novel coronavirus/Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by reverse transcription-loop-mediated isothermal amplification. [PloS one, 2020]

real-time reverse-transcriptase polymerase chain reaction (rRT-PCR), viral culture, and sequencing.

... in which assenting residents of the facility underwent nasopharyngeal and oropharyngeal testing for SARS-CoV-2, including real-time reverse-transcriptase polymerase chain reaction (rRT-PCR), viral culture, and sequencing. Symptoms that had been present during the preceding 14 days were recorded. Asymptomatic residents who ...

Ref: Presymptomatic SARS-CoV-2 Infections and Transmission in a Skilled Nursing Facility [N Engl J Med, 2020]

Mass polymerase chain reaction (PCR) screening of 300 people was undertaken in an Italian town and all positive cases were isolated.

... and isolation of asymptomatic individuals has the potential to substantially reduce the prevalence of COVID-19. Mass polymerase chain reaction (PCR) screening of 300 people was undertaken in an Italian town and all positive cases were isolated. As a result, people with COVID-19 symptoms reduced by 90% in 10 days (2) . ...

Ref: A deep dive into testing and management of COVID-19 for Australian high performance and professional sport [J Sci Med Sport, 2020-05-07]

A multi-tiered, diagnostic strategy incorporating a rapid host immune response assay as a screening test;

... the ability to efficiently screen patients, hospitals are overwhelmed, potentially delaying treatment for other emergencies. A multi-tiered, diagnostic strategy incorporating a rapid host immune response assay as a screening test; molecular confirmatory testing and rapid IgM/IgG testing to assess benefit from quarantine/further testing and provide ...

Ref: Multi-Tiered Screening and Diagnosis Strategy for COVID-19: A Model for Sustainable Testing Capacity in Response to Pandemic. [Annals of medicine, 2020-05-06]

We aimed to evaluate the antibodies-based and nucleic acid-based tests (NAT) for SARS-CoV-2-infected patients.

... However, it is urgent to need the rapid and accurate detection for SARS-CoV-2 infection diagnosis. We aimed to evaluate the antibodies-based and nucleic acid-based tests (NAT) for SARS-CoV-2-infected patients. Method: We retrospectively and observationally studied 133 patients diagnosed with SARS-CoV-2 and admitted in Renmin ...

Ref: The comparative superiority of IgM-IgG antibody test to real-time reverse transcriptase PCR detection for SARS-CoV-2 infection diagnosis [Annals of medicine, 2020-03-30]

three lateral flow rapid tests were performed.

... quantitative automated immunoassays (Maglumi™2019-n-Cov IgG and IgM and Euroimmun Anti-SARS-CoV-2 IgG and IgA assays) and three lateral flow rapid tests were performed. This retrospective study included 200 residual sera from patients and healthy volunteers. Case serum samples ...

Ref: Evaluation of two automated and three rapid lateral flow immunoassays for the detection of anti-SARS-CoV-2 antibodies [J Clin Virol, 2020]

Rapid and accurate tests that detect IgM and IgG antibodies to SARS-CoV-2 proteins

Ref: Proteome-wide analysis of differentially-expressed SARS-CoV-2 antibodies in early COVID-19 infection [J Clin Virol, 2020-04-20]

Many serological tests are available and require urgent independent validation.

... in many countries already, the current challenge is to assess population immunity on large scale. Many serological tests are available and require urgent independent validation. Here we report performance characteristics of Orient Gene (OG) COVID-19 IgG/IgM Rapid Test Cassette compare ...

Ref: Evaluation of COVID-19 IgG/IgM Rapid Test from Orient Gene Biotech. [Journal of clinical microbiology, 2020-06-09]

Current serological tests developed for SARS-CoV-2 rely on traditional technologies such as enzyme-linked immunosorbent assays (ELISA) and lateral flow assays,

... prevalence of infection, as well as ascertain individuals who may be protected from future infection. Current serological tests developed for SARS-CoV-2 rely on traditional technologies such as enzyme-linked immunosorbent assays (ELISA) and lateral flow assays, which may lack scalability to meet the demand of hundreds of millions of antibody tests ...

Ref: A rapid, point of care red blood cell agglutination assay for detecting antibodies against SARS-CoV-2 [bioRxiv, 2020-05-13]

screening for COVID-19 is performed by checking for high fever in individuals coming through airports and border controls using thermal screening guns.

... including strict security screening for passengers and baggage at all commercial airports 41 . Currently, screening for COVID-19 is performed by checking for high fever in individuals coming through airports and border controls using thermal screening guns. A nanosensor diagnostic platform is in development to test for this virus, which will replace ...

Ref: Current and emerging diagnostic tests available for the novel COVID-19 global pandemic [AAS Open Res, 2020-04-24]

Commercial and non-commercial serological tests are currently under development.

Ref: Development and Multicenter Performance Evaluation of The First Fully Automated SARS-CoV-2 IgM and IgG Immunoassays [AAS Open Res, 2020-04-21]

Preferably, the performance of serological tests for a novel virus should be determined with clinical specimens against a gold standard, i.e. virus neutralisation.

... urgent need for reliable high-throughput serological assays for the management of the ongoing COVID-19 pandemic. Preferably, the performance of serological tests for a novel virus should be determined with clinical specimens against a gold standard, i.e. virus neutralisation. We compared the performance of six commercial immunoassays for the detection of SARS-CoV-2 IgG, IgA ...

Ref: Performance of six SARS-CoV-2 immunoassays in comparison with microneutralisation [J Clin Virol, 2020-06-15]

A convenience sample of specimens that tested negative for influenza virus was tested for SARS-CoV-2 RNA.

... breath) who had no known risk for COVID-19 were identified at participating urgent care centers. A convenience sample of specimens that tested negative for influenza virus was tested for SARS-CoV-2 RNA. Among 226 patients who met the inclusion criteria, 23% had positive test results for influenza. ...

Ref: Rapid Sentinel Surveillance for COVID-19 — Santa Clara County, California, March 2020 [MMWR Morb Mortal Wkly Rep, 2020-04-10]

This study was conducted to evaluate the diagnostic performance of an immunochromatography-based assay of human serum for COVID-19.

Ref: Diagnostic performance of immunochromatography assay for rapid detection of IgM and IgG in coronavirus disease 2019 [J. med. virol, 2020]

The Xpert test provided sensitive and accurate detection of SARS-CoV-2 in a variety of upper and lower respiratory tract specimens.

... tie-breaker NAAT resolved all but three of the discordant results in favor the Xpert test.Conclusions. The Xpert test provided sensitive and accurate detection of SARS-CoV-2 in a variety of upper and lower respiratory tract specimens. The high sensitivity and fast time to results of approximately 45 minutes may impact patient ...

Ref: Multicenter Evaluation of the Cepheid Xpert Xpress SARS-CoV-2 Test. [Journal of clinical microbiology, 2020-05-04]

A multi-tiered, diagnostic strategy incorporating a rapid host immune response assay as a screening test; molecular confirmatory testing and rapid IgM/IgG testing

... the ability to efficiently screen patients, hospitals are overwhelmed, potentially delaying treatment for other emergencies. A multi-tiered, diagnostic strategy incorporating a rapid host immune response assay as a screening test; molecular confirmatory testing and rapid IgM/IgG testing to assess benefit from quarantine/further testing and provide information on population exposure/herd immunity would efficiently ...

Ref: Multi-Tiered Screening and Diagnosis Strategy for COVID-19: A Model for Sustainable Testing Capacity in Response to Pandemic. [Annals of medicine, 2020-05-06]

This study was conducted to evaluate the diagnostic performance of an immunochromatography‐based assay of human serum for COVID‐19.

Ref: Diagnostic performance of immunochromatography assay for rapid detection of IgM and IgG in coronavirus disease 2019 [J Med Virol, 2020-06-09]

In the present study, we evaluated a commercially available assay, the COVID-19 IgG/IgM Rapid Test Cassette

... In the present study, we evaluated a commercially available assay, the COVID-19 IgG/IgM Rapid Test Cassette (Zhejiang Orient Gene Biotech Co Ltd, Huzhou, Zhejiang, China), developed for detection of SARS-CoV-2-specific antibodies. ...

Ref: Evaluation of a COVID-19 IgM and IgG rapid test; an efficient tool for assessment of past exposure to SARS-CoV-2 [Infect Ecol Epidemiol, 2020-04-14]

a test for the rapid detection of combined IgG and IgM antibodies to SARS-CoV-2 in human blood, serum/plasma has become available.

... Recently, a test for the rapid detection of combined IgG and IgM antibodies to SARS-CoV-2 in human blood, serum/plasma has become available. 6 As soon as the kits were commercially available, we assessed the reliability and usefulness of the 2019-nCoV IgG/IgM Antibody Rapid 2) 7 patients with suspected Covid 19 who ...

Ref: Reliability and usefulness of a rapid IgM‐IgG antibody test for the diagnosis of SARS-CoV-2 infection: a preliminary report. [J Infect, 2020-04-23]

Previous studies have evaluated the possible role of a quick detection approach targeting viral IgM or IgG antibody using different methods.

... Previous studies have evaluated the possible role of a quick detection approach targeting viral IgM or IgG antibody using different methods. Results have been conflicting with respect to the sensitivity of this approach. Thus, antibody determination is not advocate for SARS-CoV 2 infection diagnosis. [2] [3] [4] The knowledge of antibody"s ...

Ref: Qualitative serology in patients recovered from SARS CoV 2 infection [J Infect, 2020-05-28]

IgM-, IgA- and total antibody-ELISAs increased in sensitivity to >80% between the 6th and 10th day post symptom onset.

... onset. While test sensitivities were low (<40%) within the first 5 days post disease onset, IgM-, IgA- and total antibody-ELISAs increased in sensitivity to >80% between the 6th and 10th day post symptom onset. The evaluated tests (including IgG and rapid tests) provided positive results in all patients at ...

Ref: Performance of SARS-CoV-2 antibody assays in different stages of the infection: Comparison of commercial ELISA and rapid tests [J. infect. dis, 2020]

we developed an IgG-IgM combined antibody test for COVID-19 infection ( Figure 1 ).

... shown in Table 3 To make the kit suitable for different stages of the disease, we developed an IgG-IgM combined antibody test for COVID-19 infection ( Figure 1 ). It was also been confirmed that the detection sensibility was higher in IgG-IgM combined antibody ...

Ref: Development and clinical application of a rapid IgM‐IgG combined antibody test for SARS‐CoV‐2 infection diagnosis [J Med Virol, 2020-04-13]

Molecular assays are the preferred method to detect a broad range of pathogens with high sensitivity and specificity.

... of infectious disease surveillance is a critical component in the response to novel health threats. Molecular assays are the preferred method to detect a broad range of pathogens with high sensitivity and specificity. The implementation of molecular assay testing in a rapidly evolving public health emergency can be ...

Ref: Increasing testing throughput and case detection with a pooled-sample Bayesian approach in the context of COVID-19 [bioRxiv, 2020-04-05]

early reporting, early isolation, early diagnosis and early management have been proved to be useful in the course of COVID-19 patients [4] .

Early detection, early reporting, early isolation, early diagnosis and early management have been proved to be useful in the course of COVID-19 patients [4] .

Ref: Diagnostic Indexes of a Rapid IgG/IgM Combined Antibody Test for SARS-CoV-2 [bioRxiv, 2020-03-30]

The rapid SARS-CoV-2 IgG-IgM combined antibody test kits were tested at eight hospitals and Chinese CDC laboratories in different provinces,

... The rapid SARS-CoV-2 IgG-IgM combined antibody test kits were tested at eight hospitals and Chinese CDC laboratories in different provinces, with a total of 397 clinical positive and 128 clinical negative patient blood samples. The test data was collected and analyzed. The specificity and sensitivity of the rapid test kits ...

Ref: Development and clinical application of a rapid IgM‐IgG combined antibody test for SARS‐CoV‐2 infection diagnosis [J Med Virol, 2020-04-13]

this test represents an important immunological tool aimed at identifying the precise phase of the infection in order to undertake a more appropriate pharmacological treatment.

... paper we also described the importance of sensitive and specific rapid test for SARS-CoV-2. Indeed, this test represents an important immunological tool aimed at identifying the precise phase of the infection in order to undertake a more appropriate pharmacological treatment. Lastly, we provided an overview of pharmacological treatments aimed to reduce inflammatory processes underlying the ...

Ref: SARS-Cov-2 infection: Response of human immune system and possible implications for the rapid test and treatment [Int Immunopharmacol, 2020]

Patients (n=102) with a positive SARS-CoV-2 RT-PCR were tested.

... Orient Gene (OG) COVID-19 IgG/IgM Rapid Test Cassette compare it Abbott SARS-CoV-2 IgG immunoassay (ASIA). Patients (n=102) with a positive SARS-CoV-2 RT-PCR were tested. They were asymptomatic (n=2), had mild (n=37) or severe symptoms requiring hospitalization in medical (n=35) ...

Ref: Evaluation of COVID-19 IgG/IgM Rapid Test from Orient Gene Biotech [J. clin. microbiol, 2020]

It detects coronavirus ribonucleic acid (RNA) on polymerase chain reaction (PCR).

The first COVID-19 test is performed on swabs from the nasopharynx or sometimes tracheobronchial tract, oropharynx or sputum. It detects coronavirus ribonucleic acid (RNA) on polymerase chain reaction (PCR).

Ref: Keys for analysis of diagnostic and serologic tests for CoV-2 [Eur Ann Otorhinolaryngol Head Neck Dis, 2020-05-27]

We have thus tested 4 immunochromatographic tests for the detection of antibodies to SARS-CoV-2.

... tests are or will be very soon available but need to be evaluated and validated. We have thus tested 4 immunochromatographic tests for the detection of antibodies to SARS-CoV-2. In addition, we assessed the kinetics of antibody appearance using these assays in 22 patients ...

Ref: Dynamic profile for the detection of anti-SARS-CoV-2 antibodies using four immunochromatographic assays [J Infect, 2020-05-07]

COVID-19 (Coronavirus infectious disease 19) epidemics spread from Wuhan, China, to all over the world, including Italy.

From late December 2019 COVID-19 (Coronavirus infectious disease 19) epidemics spread from Wuhan, China, to all over the world, including Italy. This article is protected by copyright. All rights reserved.

Ref: Performance of VivaDiag COVID‐19 IgM/IgG Rapid Test is inadequate for diagnosis of COVID‐19 in acute patients referring to emergency room department [J Med Virol, 2020-04-08]

NG-Test® IgG-IgM COVID-19 rapid test cassettes (NG Biotech Laboratoires) were kindly provided by the manufacturer.

This research did not receive any specific grant from funding agencies. NG-Test® IgG-IgM COVID-19 rapid test cassettes (NG Biotech Laboratoires) were kindly provided by the manufacturer.

Ref: Assessment of SARS-CoV-2 serological tests for the diagnosis of COVID-19 through the evaluation of three immunoassays: two automated immunoassays (Euroimmun and Abbott) and one rapid lateral flow immunoassay (NG Biotech) [J Clin Virol, 2020-06-15]

the development of immunoassays to help determine exposure and potentially predict immunity has become a pressing priority.

... As the Coronavirus 2019 (COVID-19) pandemic evolves, the development of immunoassays to help determine exposure and potentially predict immunity has become a pressing priority. In this report we present the performance of the EUROIMMUN enzyme-linked immunosorbent assay (ELISA) for semi-quantitative detection of IgA and IgG antibodies in ...

Ref: Evaluation of the EUROIMMUN Anti-SARS-CoV-2 ELISA Assay for detection of IgA and IgG antibodies [J Clin Virol, 2020-05-23]

testing for infection enables frontline healthcare and other key workers to safely go to work.

... [10]. As well as facilitating index case isolation and contact tracing in the early stages, testing for infection enables frontline healthcare and other key workers to safely go to work. Effective antibody tests could enable billions globally to safely return to work from lockdowns much ...

Ref: cord_uid o5enerl8 Group Testing for SARS-CoV-2: Forward to the P... o5enerl8 Group Testing for SARS-CoV-2: Forward to the P... Name: title, dtype: object [J Clin Virol, cord_uid o5enerl8 2020-04-28 o5enerl8 2020-04-28 Name: publish_time, dtype: object]

The most common sample types being tested are swabs taken from the nasopharynx and/or oropharynx,

... that detect viral RNA are contingent on viral RNA being present in the sample collected. The most common sample types being tested are swabs taken from the nasopharynx and/or oropharynx, with the former considered somewhat more sensitive than the latter (2); if both are collected, ...

Ref: Report from the American Society for Microbiology COVID-19 International Summit, 23 March 2020: Value of Diagnostic Testing for SARS–CoV-2/COVID-19 [mBio, 2020-03-26]

the need to accelerate progress in diagnostics, serological tests have been developed.

Ref: Diagnostic accuracy of an automated chemiluminescent immunoassay for anti‐SARS‐CoV‐2 IgM and IgG antibodies: an Italian experience [J Med Virol, 2020-05-10]

Positive rates for SARS-CoV-2 did not differ by sex or age.

... COVID-19, 11 (7.3%, 95% confidence interval: 3.1%-11.6%) tested positive again for SARS-CoV-2 in throat swabs. Positive rates for SARS-CoV-2 did not differ by sex or age. Characteristics were similar between those positive and those not ( Table 1 ). There were ...

Ref: Recurrent positive SARS‐CoV‐2: Immune certificate may not be valid [J Med Virol, 2020-06-09]

Till date, various immunological and nucleic acid amplification diagnostic tests are developed and easily available.

... urgent need to have accurate, rapid, readily available and reliable diagnostic test for SARS-CoV-2 infection. Till date, various immunological and nucleic acid amplification diagnostic tests are developed and easily available. Various integrated point-of-care molecular devices are currently under development and some are available to provide ...

Ref: Laboratory diagnosis of SARS-CoV-2 - A review of current methods [J Infect Public Health, 2020-06-07]

The gold standard is a quantitative polymerase chain reaction (qPCR)–based system taking several hours to confirm positivity.

... SARS-CoV2 outbreak, countless tests need to be performed on potential symptomatic individuals, contacts and travellers. The gold standard is a quantitative polymerase chain reaction (qPCR)–based system taking several hours to confirm positivity. For effective public health containment measures, this time span is too long. We therefore evaluated ...

Ref: Rapid point-of-care testing for SARS-CoV-2 in a community screening setting shows low sensitivity [Public Health, 2020-04-18]

Laboratory confirmation of SARS-CoV-2 infection was based on RT-qPCR-positive results 34 .

... serological detection tools have been rapidly developed 8, 18, 26, [30] [31] [32] [33] . Laboratory confirmation of SARS-CoV-2 infection was based on RT-qPCR-positive results 34 . However, molecular tests carried out through swabs can also be negative in people who harbor ...

Ref: SARS-CoV-2 infection serology: a useful tool to overcome lockdown? [Cell Death Discov, 2020-05-26]

SARS-CoV-2 serological assays are urgently needed for rapid diagnosis, contact tracing and for epidemiological studies.

Ref: Clinical performance of different SARS-CoV-2 IgG antibody tests. [Journal of medical virology, 2020-06-08]

We evaluated one of the available serological tests, the Prima Lab Covid-19 IgG/IgM Rapid Tests, on 739 volunteers.

... subjects in the general population is of paramount relevance for the control of the disease. We evaluated one of the available serological tests, the Prima Lab Covid-19 IgG/IgM Rapid Tests, on 739 volunteers. We first assessed the test"s reproducibility by administering it twice on the same day on ...

Ref: Evaluating the adequacy of Prima Covid-19 IgG/IgM Rapid Test for the assessment of exposure to SARS-CoV-2 virus [Journal of medical virology, 2020-06-03]

On March 13, CDC performed symptom assessments and SARS-CoV-2 testing for 76 (93%) of the 82 facility A residents

... seven residents of this second facility were symptomatic and had positive test results for SARS-CoV-2. On March 13, CDC performed symptom assessments and SARS-CoV-2 testing for 76 (93%) of the 82 facility A residents to evaluate the utility of symptom screening for identification of COVID-19 in SNF residents. Residents ...

Ref: Asymptomatic and Presymptomatic SARS-CoV-2 Infections in Residents of a Long-Term Care Skilled Nursing Facility - King County, Washington, March 2020 [MMWR Morb Mortal Wkly Rep, 2020]

several easy to perform rapid antigen detection tests were developed and recommended in some countries as the first line of diagnostic.

... of special equipment and skilled staff limit the use of these time-consuming molecular techniques. Recently, several easy to perform rapid antigen detection tests were developed and recommended in some countries as the first line of diagnostic. OBJECTIVES: The aim of this study was to evaluate the performances of the Coris COVID-19 ...

Ref: Low performance of rapid antigen detection test as frontline testing for COVID-19 diagnosis [J Clin Virol, 2020]

These included three enzyme-linked immunosorbent assays (ELISAs) and six point-of-care (POC) lateral flow tests.

... epidemiological studies. This study evaluated the sensitivity and specificity of nine commercially available serological tests. These included three enzyme-linked immunosorbent assays (ELISAs) and six point-of-care (POC) lateral flow tests. The assays were validated using serum samples from: i) SARS-CoV-2 PCR-positive patients with a documented ...

Ref: Evaluation of nine commercial SARS-CoV-2 immunoassays [J Clin Virol, 2020-04-10]

Serology testings targeting on viral-induced antibodies are given different information as those for viral RNA and proteins from SARS-CoV-2.

... a transducer. Many of the entrepreneurs may take this opportunity to fasten their product development. Serology testings targeting on viral-induced antibodies are given different information as those for viral RNA and proteins from SARS-CoV-2. Although the protein testing method is similar, the targets are not part of the virus ...

Ref: Molecular Targets for the Testing of COVID‐19 [Biotechnol J, 2020-05-18]

Testing of specific antibodies of SARS-CoV-2 in patient blood

Testing of specific antibodies of SARS-CoV-2 in patient blood is a good choice for rapid, simple, highly sensitive diagnosis of COVID-19.

Ref: Development and clinical application of a rapid IgM‐IgG combined antibody test for SARS‐CoV‐2 infection diagnosis [J Med Virol, 2020-04-13]

Viral antigen was detected by a rapid antigen immunochromatographic assay.

... cobas SARS-CoV-2 high-throughput system, three direct RT-qPCR kits, and reverse-transcription loop mediated isothermal amplification (RT-LAMP). Viral antigen was detected by a rapid antigen immunochromatographic assay. Results Of the 103 samples, viral RNA was detected in 50.5-81.6% of the specimens by ...

Ref: Clinical evaluation of self-collected saliva by RT-qPCR, direct RT-qPCR, RT-LAMP, and a rapid antigen test to diagnose COVID-19 [J Med Virol, 2020-06-08]

METHODS: We evaluated a lateral flow immunoassay (AllTest COVID-19 IgG/IgM) which detects IgG and IgM antibodies.

... study was to analyze the diagnostic performance of one serologic rapid test in COVID-19 patients. METHODS: We evaluated a lateral flow immunoassay (AllTest COVID-19 IgG/IgM) which detects IgG and IgM antibodies. We validated the serologic test using serum samples from 100 negative patients (group 1) and ...

Ref: Alltest rapid lateral flow immunoassays is reliable in diagnosing SARS-CoV-2 infection from 14 days after symptom onset: A prospective single-center study [J Clin Virol, 2020]

assays based on the reverse-transcription polymerase chain reaction (RT-PCR) in respiratory samples are the gold standard for COVID-19 diagnosis.

... laboratory testing is an essential step in the management of the COVID-19 outbreak. To date, assays based on the reverse-transcription polymerase chain reaction (RT-PCR) in respiratory samples are the gold standard for COVID-19 diagnosis. Unfortunately, RT-PCR has several practical limitations. Consequently, alternative diagnostic methods are urgently required, both for ...

Ref: Point-of-Care Diagnostic Tests for Detecting SARS-CoV-2 Antibodies: A Systematic Review and Meta-Analysis of Real-World Data [J Clin Virol, 2020]

The evaluated rapid test is an IgG/IgM-based test for SARS-CoV-2 with a time to result of 20 min.

... a rapid test. Ten previously diagnosed individuals with known SARS-CoV-2 infection were also analysed. METHODS: The evaluated rapid test is an IgG/IgM-based test for SARS-CoV-2 with a time to result of 20 min. Two drops of blood are needed for the test performance. RESULTS: Of 49 individuals, 22 ...

Ref: Rapid point-of-care testing for SARS-CoV-2 in a community screening setting shows low sensitivity [Public Health, 2020]