¿Hay pruebas serológicas que detecten anticuerpos contra el coronavirus?

(Are there serological tests that detect antibodies to coronavirus?)

Primeras 5 respuestas:

1. Serological tests rely on detection of specific anti-viral antibodies (IgM, IgG, IgA or total antibody) in patient serum, plasma or whole blood.


2. enzyme-linked immunosorbent assay (ELISA) and lateral flow immunoassay.


3. Serological tests detect antibodies against the suspected pathogen, which are produced by an individual's immune system.


4. lateral flow immunochromatography or enzymelinked immunosorbent assays (ELISA).


5. Serological testing consisted of an enzyme linked immunosorbent assay designed to detect IgM and IgG antibodies against the N protein of SARS-CoV-2.

Serological tests rely on detection of specific anti-viral antibodies (IgM, IgG, IgA or total antibody) in patient serum, plasma or whole blood.

... been rapid development of serological assays for COVID-19 in a number of countries (5) . Serological tests rely on detection of specific anti-viral antibodies (IgM, IgG, IgA or total antibody) in patient serum, plasma or whole blood. The broad array of serological tests now available vary both in analytical performance and in ...

Ref: Evaluation of serological tests for SARS-CoV-2: Implications for serology testing in a low-prevalence setting [Public Health, 2020-06-03]

enzyme-linked immunosorbent assay (ELISA) and lateral flow immunoassay.

... Identification of individuals who have developed antibodies to the SARS-CoV-2 virus requires serological tests, including enzyme-linked immunosorbent assay (ELISA) and lateral flow immunoassay. This report also provides an overview of current development in COVID-19 diagnostic techniques and products ...

Ref: Assay Techniques and Test Development for COVID-19 Diagnosis [ACS Cent Sci, 2020-04-30]

Serological tests detect antibodies against the suspected pathogen, which are produced by an individual's immune system.

... relating to the suspected pathogen is indicative of an active infection with the suspected pathogen. Serological tests detect antibodies against the suspected pathogen, which are produced by an individual"s immune system. A positive serological test result indicates recent exposure to the suspected pathogen but cannot be ...

Ref: COVID-19 Special Column: Principles Behind the Technology for Detecting SARS-CoV-2, the Cause of COVID-19 [Hawaii J Health Soc Welf, 2020]

lateral flow immunochromatography or enzymelinked immunosorbent assays (ELISA).

... tests have recently become available. Serologic tests for antibodies to SARS-CoV-2 are typically based on lateral flow immunochromatography or enzymelinked immunosorbent assays (ELISA). Currently available tests predominantly target antibodies to 1 of 2 main surface proteins of the ...

Ref: Double-Edged Spike—Are SARS-CoV-2 Serologic Tests Safe Right Now? [Lab Med, 2020-04-23]

Serological testing consisted of an enzyme linked immunosorbent assay designed to detect IgM and IgG antibodies against the N protein of SARS-CoV-2.

... healthcare staff and patients with a variety of diseases from bacterial pneumonia to lung cancer. Serological testing consisted of an enzyme linked immunosorbent assay designed to detect IgM and IgG antibodies against the N protein of SARS-CoV-2. Serial examination of COVID-19 patients resulted in IgM seroreactivity by day 4 post symptom onset, ...

Ref: Role of serology in the COVID-19 pandemic [Clin Infect Dis, 2020-05-01]

For antibody test, diverse assays targeting different antigens, and collecting paired samples are needed.

... salt to inactivate virus as well as protect RNA; setting proper positive, negative and inhibition controls to ensure high-quality results; simultaneously amplifying human RNase P gene to avoid false-negative results. For antibody test, diverse assays targeting different antigens, and collecting paired samples are needed. ...

Ref: Laboratory testing of SARS-CoV, MERS-CoV, and SARS-CoV-2 (2019-nCoV): Current status, challenges, and countermeasures [Rev Med Virol, 2020]

Accurate serologic tests to detect host antibodies to severe acute respiratory syndrome-related coronavirus-2

... Accurate serologic tests to detect host antibodies to severe acute respiratory syndrome-related coronavirus-2 (SARS-CoV-2) will be critical for the public health response to the coronavirus disease 2019 pandemic. Many use cases are envisaged, including complementing molecular methods for diagnosis of active disease and ...

Ref: Serodiagnostics for Severe Acute Respiratory Syndrome-Related Coronavirus-2: A Narrative Review [Ann. intern. med, 2020]

A newly-developed ELISA assay for IgM and IgG antibodies against N protein of SARS-CoV-2

... In this study, we aimed to evaluate the diagnostic value of serological assay for SARS-CoV-2. A newly-developed ELISA assay for IgM and IgG antibodies against N protein of SARS-CoV-2 was used to screen the serums of 238 admitted hospital patients between February 6 and ...

Ref: A preliminary study on serological assay for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in 238 admitted hospital patients [Microbres infect, 2020]

The assay was able to detect SARS-CoV-2 antibody on day one after the onset of COVID-19 disease.

... against 69 samples from hospitalized and/or recovered COVID-19 patients. The overall accuracy rate reached 97.3%. The assay was able to detect SARS-CoV-2 antibody on day one after the onset of COVID-19 disease. The average antibody levels increased during the hospitalization and after been discharged for two weeks. ...

Ref: Early detection of SARS-CoV-2 antibodies in COVID-19 patients as a serologic marker of infection [Clin. infect. dis, 2020]

SARS-CoV-2-associatedimmunoglobulinswere assayed by utilizing a rapid serological colorimetric test.

... insight into this aspect of the epidemic, we tested525 asymptomatic workers of our Cancer Institute where immuno-depressed patients are extremely frequent and sporadic COVID-19 positive cases had recently been reported. SARS-CoV-2-associatedimmunoglobulinswere assayed by utilizing a rapid serological colorimetric test. ...

Ref: RAPID SEROLOGICAL TESTS HAVE A ROLE IN ASYMPTOMATIC HEALTH WORKERS COVID-19 SCREENING [Clin. infect. dis, 2020-04-17]

We have thus tested 4 immunochromatographic tests for the detection of antibodies to SARS-CoV-2.

... tests are or will be very soon available but need to be evaluated and validated. We have thus tested 4 immunochromatographic tests for the detection of antibodies to SARS-CoV-2. In addition, we assessed the kinetics of antibody appearance using these assays in 22 patients ...

Ref: Dynamic profile for the detection of anti-SARS-CoV-2 antibodies using four immunochromatographic assays [J. infect, 2020]

lateral flow immunochromatography or enzyme-linked immunosorbent assays (ELISA).

... tests have recently become available. Serologic tests for antibodies to SARS-CoV-2 are typically based on lateral flow immunochromatography or enzyme-linked immunosorbent assays (ELISA). Currently available tests predominantly target antibodies to 1 of 2 main surface proteins of the ...

Ref: Double-Edged Spike: Are SARS-CoV-2 Serologic Tests Safe Right Now? [Am J Clin Pathol, 2020-04-23]

CEFA and MIA detected SARS-CoV-2 antibodies in 26.2% and 26.3%, respectively, of ED patients.

... were evaluated. RESULTS: Agreement of CEFA and MIA was 90.4%-94.5% (Kappa: 0.81-0.89) in 302 samples. CEFA and MIA detected SARS-CoV-2 antibodies in 26.2% and 26.3%, respectively, of ED patients. Detection rates increased over time reaching 100% after 21 days post-symptom onset. Longitudinal antibody kinetic ...

Ref: SARS-CoV-2 antibody characterization in emergency department, hospitalized and convalescent patients by two semi-quantitative immunoassays [Clin Chim Acta, 2020-06-04]

The test used to detect SARS-CoV-2 IgG antibodies is the LIAISON® SARS-CoV-2 test.

... asymptomatic case contacts identified through an epidemiological investigation without history of a swab for SARS-CoV-2. The test used to detect SARS-CoV-2 IgG antibodies is the LIAISON® SARS-CoV-2 test. The LIAISON® SARS-CoV-2 test employs magnetic beads coated with S1 & S2 antigens. The antigens ...

Ref: Probability of symptoms and critical disease after SARS-CoV-2 infection [Clin Chim Acta, 2020-06-15]

anti-SARS CoV-2 antibodies IgM and IgG.

... patients and 64 patients from a control group were tested by iFlash1800 CLIA analyzer for anti-SARS CoV-2 antibodies IgM and IgG. All COVID-19 patients were hospitalized in San Giovanni di Dio Hospital (Florence, Italy) and had ...

Ref: Diagnostic accuracy of an automated chemiluminescent immunoassay for anti-SARS-CoV-2 IgM and IgG antibodies: an Italian experience [J. med. virol, 2020]

Neutralising antibodies with neutralisation test (NT) titres ≥20 were detected in 26 of 42 serum samples (61.9%).

... Neutralising antibodies with neutralisation test (NT) titres ≥20 were detected in 26 of 42 serum samples (61.9%). Besides the RT-PCR confirmed SARS-CoV-2 cases (n=8), 13 out of 19 symptomatic (68.4%) and 5 of 15 asymptomatic (33.3%) individuals had neutralising antibodies (table S2) . Neutralising antibody levels in ...

Ref: Sensitivity of commercial Anti-SARS-CoV-2 serological assays in a high-prevalence setting [J. med. virol, 2020-06-12]

We developed serologic assays for detection of SARS-CoV-2 neutralizing, spike protein-specific, and nucleocapsid-specific antibodies.

... Validated serologic assays are needed for contact tracing, identifying the viral reservoir, and epidemiologic studies. We developed serologic assays for detection of SARS-CoV-2 neutralizing, spike protein-specific, and nucleocapsid-specific antibodies. Using serum samples from patients with PCR-confirmed SARS-CoV-2 infections, other coronaviruses, or other respiratory pathogenic ...

Ref: Severe Acute Respiratory Syndrome Coronavirus 2-Specific Antibody Responses in Coronavirus Disease 2019 Patients. [Emerging infectious diseases, 2020-04-08]

serological assays to detect antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are now becoming available from numerous diagnostic manufacturers.

... requirements and concerns regarding their diagnostic accuracy in clinical settings. In addition to molecular testing, serological assays to detect antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are now becoming available from numerous diagnostic manufacturers. In both cases, the lack of peer-reviewed data and regulatory oversight, combined with general misconceptions ...

Ref: Molecular, serological, and biochemical diagnosis and monitoring of COVID-19: IFCC taskforce evaluation of the latest evidence. [Clinical chemistry and laboratory medicine, 2020-05-27]

The purpose of this study was to assess the performance of five immunoassays for the detection of SARS-CoV-2 antibodies.

... INTRODUCTION: Several SARS-CoV-2 immunoassays have been developed recently. The purpose of this study was to assess the performance of five immunoassays for the detection of SARS-CoV-2 antibodies. METHODS: Two quantitative automated immunoassays (Maglumi™2019-n-Cov IgG and IgM and Euroimmun Anti-SARS-CoV-2 IgG and IgA assays) and three lateral flow rapid tests ...

Ref: Evaluation of two automated and three rapid lateral flow immunoassays for the detection of anti-SARS-CoV-2 antibodies [J Clin Virol, 2020]

Antibody tests are blood tests that detect antibodies or immunoglobins (Ig) that are produced as human immune response to SARS-CoV-2 infection.

... Antibody tests are blood tests that detect antibodies or immunoglobins (Ig) that are produced as human immune response to SARS-CoV-2 infection. A positive result suggests that the individual has potentially been exposed to SARS-CoV-2. When IgM antibodies are present, they can indicate an active or recent infection. IgG antibodies show up ...

Ref: Antibody Testing For Covid-19: Can It Be Used As A Screening Tool In Areas With Low Prevalence? [Am J Clin Pathol, 2020-05-15]

The commercially available assays examined in our study, generated consistent results regarding the detection of SARS-CoV-2-IgG antibodies.

... higher sensitivity a couple of weeks after infection (5, 6) . The commercially available assays examined in our study, generated consistent results regarding the detection of SARS-CoV-2-IgG antibodies. The sensitivity (without differentiating the timepoint of sampling) varied within the group of assays using the same antigen as ...

Ref: Brief clinical evaluation of six high-throughput SARS-CoV-2 IgG antibody assays [J Clin Virol, 2020-06-01]

Across all 160 participants, 159 had detectable anti-SARS-CoV-2 antibodies by S-Flow (99.4% sensitivity).

... three assays and categorized by the delay between onset of symptoms and collection of samples. Across all 160 participants, 159 had detectable anti-SARS-CoV-2 antibodies by S-Flow (99.4% sensitivity). The only participant whose serology was negative with all assays was a 58-yearold male with ...

Ref: Serologic responses to SARS-CoV-2 infection among hospital staff with mild disease in eastern France [J Clin Virol, 2020-05-22]

IgM, IgA and IgG antibodies were detectable in some 115 patients as early as day 1 after onset of symptoms.

... different isotypes of SARS-CoV-2 specific immunoglobulins is comparable to other 114 coronavirus infections 2-10 . IgM, IgA and IgG antibodies were detectable in some 115 patients as early as day 1 after onset of symptoms. The interquartile ranges of the 116 first antibody detection for IgM and IgA are between ...

Ref: SARS-CoV-2 Antibody Testing – Questions to be asked [J Allergy Clin Immunol, 2020-05-29]

SARS-CoV-2 antibody assays are needed for serological surveys and as a complement to molecular tests to confirm COVID-19.

... OBJECTIVES: SARS-CoV-2 antibody assays are needed for serological surveys and as a complement to molecular tests to confirm COVID-19. However, the kinetics of the humoral response against SARS-CoV-2 remains poorly described and relies on the performance of the different serological tests. METHODS: In this study, we evaluated the ...

Ref: Detection of SARS-CoV-2 antibodies using commercial assays and seroconversion patterns in hospitalized patients [J. infect, 2020]

We also tested the cross-reactivity of IgG rS1-ELISA-reactive samples with the four circulating human coronaviruses using ELISA and IIFT.

... followed by evaluation of reactive samples using whole-virus indirect immunofluorescence assays (IgM-and IgG-IIFT) and ppNT. We also tested the cross-reactivity of IgG rS1-ELISA-reactive samples with the four circulating human coronaviruses using ELISA and IIFT. This study targeted three groups: (i) low-risk group constituted of 4719 samples obtained from blood ...

Ref: Comparative Serological Study for the Prevalence of Anti-MERS Coronavirus Antibodies in High- and Low-Risk Groups in Qatar [J Immunol Res, 2019-02-18]

The humoral response to some SARS-associated coronavirus shows a simultaneous increase in the level of all antibodies (25) .

... Most tests assessed levels of IgG and IgM simultaneously. The humoral response to some SARS-associated coronavirus shows a simultaneous increase in the level of all antibodies (25) . Also, the dual detection of IgG-IgM improves the sensibility in comparison with individual IgG or IgM antibody assay (11), suggesting a ...

Ref: Immunological assays for SARS-CoV-2: an analysis of available commercial tests to measure antigen and antibodies [J Immunol Res, 2020-04-14]

the identifications of antibodies require both acute and convalescent serum sampling.

... the isolation and application of quarantine measures. For serologic diagnosis of MERS-CoV and SARS-CoV-2 infections, the identifications of antibodies require both acute and convalescent serum sampling. This is particularly important as we witness an increased number of asymptomatic or pre-symptomatic COVID-19 ...

Ref: Serologic testing of coronaviruses from MERS-CoV to SARS-CoV-2: Learning from the past and anticipating the future [Travel Med Infect Dis, 2020-06-10]

Lateral flow chromatographic immunoassay for qualitative detection of IgG and IgM antibodies to 2019-nCoV in human whole blood, serum, or plasma specimens

... Lateral flow chromatographic immunoassay for qualitative detection of IgG and IgM antibodies to 2019-nCoV in human whole blood, serum, or plasma specimens (2019-nCoV IgG/IgM Rapid Test Cassette, Hangzhou AllTest Biotech Co, Hangzhou, China; distributed in Italy by Alifax Srl, Padova, IT). During testing, sample reacts with 2019-nCoV antigen-coated particles (recombinant N-Protein, as ...

Ref: SARS-CoV-2 infection serology: a useful tool to overcome lockdown? [Cell Death Discov, 2020-05-26]

Many serological enzyme-linked immunosorbent assays (ELISA) have been recently developed to detect anti-SARS-CoV-2 antibodies.

... Many serological enzyme-linked immunosorbent assays (ELISA) have been recently developed to detect anti-SARS-CoV-2 antibodies. However, these assays have four important limitations [4] [5] [6] [7] [8] [9] [10] [11] [12] [13] [14] [15] . First, they lack the ability to detect antibodies at early ...

Ref: Ultra-Sensitive High-Resolution Profiling of Anti-SARS-CoV-2 Antibodies for Detecting Early Seroconversion in COVID-19 Patients [medRxiv : the preprint server for health sciences, 2020-05-02]

Multiple SARS-COV-2 antibody assays have been developed and/or validated in a growing number of different individual labs.

... Multiple SARS-COV-2 antibody assays have been developed and/or validated in a growing number of different individual labs. Generally these tests show an improved sensitivity with N antigen-based tests, that IgG tests perform better when compared to IgM tests, and most show better sensitivity when the samples are ...

Ref: Performance and health economic evaluation of the Mount Sinai COVID-19 serological assay identifies modification of thresholding as necessary to maximise specificity of the assay [medRxiv : the preprint server for health sciences, 2020-06-12]

Serum levels of IgM-IgG antibodies targeting SARS-CoV-2 were tested upon patient admission.

... to fever or respiratory symptoms. Nasopharyngeal and throat swabs were used for respiratory pathogen testing. Serum levels of IgM-IgG antibodies targeting SARS-CoV-2 were tested upon patient admission. Medical history about when the clinical symptoms appeared was asked and the time interval between ...

Ref: Characteristics of patients with coronavirus disease (COVID‐19) confirmed using an IgM‐IgG antibody test [J Med Virol, 2020-05-07]

immunofluorescence assays (IFAs), enzyme-linked immunosorbent assays (ELISAs) and Western blot (WB) analysis.

During the outbreak of SARS-CoV, a wide variety of serological assays were established, including immunofluorescence assays (IFAs), enzyme-linked immunosorbent assays (ELISAs) and Western blot (WB) analysis.

Ref: Serological assays for emerging coronaviruses: Challenges and pitfalls [Virus Res, 2014-12-19]

This comprises 273 molecular assays and 287 immunoassays.

... on the 22 May 2019 contained 560 SARS-CoV-2 laboratory tests for the diagnosis of COVID-19. This comprises 273 molecular assays and 287 immunoassays. Excluding those intended for research use only, 152 of these are molecular assays and 211 ...

Ref: Testing for SARS-CoV-2 (COVID-19): a systematic review and clinical guide to molecular and serological in-vitro diagnostic assays [Reprod Biomed Online, 2020-06-14]

total antibodies (Ab), IgM and IgG against SARS-CoV-2.

... infection were enrolled. Their serial plasma samples (n=535) collected during the hospitalization were tested for total antibodies (Ab), IgM and IgG against SARS-CoV-2. The dynamics of antibodies with the disease progress was analyzed. RESULTS: Among 173 patients, the ...

Ref: Antibody responses to SARS-CoV-2 in patients of novel coronavirus disease 2019 [Clin. infect. dis, 2020]

ResultsIgG and IgM RBD ELISA, MN and PRNT90 were reliably positive after 29 days from illness onset

... from 24 patients with virologically confirmed COVID-19 and in age-stratified sera from 200 healthy controls. ResultsIgG and IgM RBD ELISA, MN and PRNT90 were reliably positive after 29 days from illness onset with no detectable cross-reactivity in age-stratified controls. We found that PRNT90 tests were more sensitive ...

Ref: Serological assays for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), March 2020 [Euro surveill, 2020]

Real-time reverse transcription-polymerase chain reactions (rRT-PCR) can detect SARS-COV-2

... virological and serological tests are essential for detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Real-time reverse transcription-polymerase chain reactions (rRT-PCR) can detect SARS-COV-2 by targeting open reading frame-1 antibodies (ORF1ab), envelope protein, nucleocapsid protein, RNA-dependent RNA polymerase genes, ...

Ref: In vitro diagnostics of coronavirus disease 2019: Technologies and application [J. microbiol. immunol. infect, 2020]

A recent study validated the VivaDiag IgM/IgG Rapid Test 37 in acute patients referring to emergency room department in Italy.

... antibody testing is not useful in the setting of an acute illness 22, 23 . A recent study validated the VivaDiag IgM/IgG Rapid Test 37 in acute patients referring to emergency room department in Italy. The sensitivity and specificity were 18.4% and 91.7%, indicating the serology This article is protected ...

Ref: An Evolving Approach to the Laboratory Assessment of COVID‐19 [J Med Virol, 2020-04-29]

We compared the performance of six commercial immunoassays for the detection of SARS-CoV-2 IgG, IgA and IgM antibodies,

... novel virus should be determined with clinical specimens against a gold standard, i.e. virus neutralisation. We compared the performance of six commercial immunoassays for the detection of SARS-CoV-2 IgG, IgA and IgM antibodies, including four automated assays [Abbott SARS-COV-2 IgG (CE marked), Diasorin Liaison® SARS-CoV-2 S1/S2 IgG (research ...

Ref: Performance of six SARS-CoV-2 immunoassays in comparison with microneutralisation [J Clin Virol, 2020-06-15]

Immunoenzymatic Assay (ELISA), Chemiluminescence, Electro Chemiluminescence, Fluorescent Lateral Flow Immunoassays and Immunochromatography.

... Background The COVID-19 serological tests for IgG and IgM have been developed with several methodologies: Immunoenzymatic Assay (ELISA), Chemiluminescence, Electro Chemiluminescence, Fluorescent Lateral Flow Immunoassays and Immunochromatography. None of these tests should be used for the diagnosis or population screening of the ...

Ref: Concordance of "rapid" serological tests and IgG and IgM chemiluminescence for SARS-COV-2 [J Clin Virol, 2020-06-03]

One Step Novel Coronavirus (COVID-19) IgM/IgG Antibody Test was used.

... and samples from health care workers belong to two Governmental hospitals and one private clinic. One Step Novel Coronavirus (COVID-19) IgM/IgG Antibody Test was used. Out of the 219 samples tested, 6 (2.74%) samples were seropositive for SARS-CoV-2. All health-care ...

Ref: Seroprevalence of antibodies against SARS-CoV-2 among public community and health-care workers in Alzintan City of Libya [J Clin Virol, 2020-05-26]

Serum samples were collected from patients who had previously tested positive for SARS-CoV-2 infection by a PCR test,

... blood samples for assay validation was reviewed and approved by Western IRB (study ID: VYR-COV-001). Serum samples were collected from patients who had previously tested positive for SARS-CoV-2 infection by a PCR test, from patients who had known exposure to individuals infected with SARS-CoV-2 and symptoms of COVID-19, ...

Ref: Development and validation of IMMUNO-COV™: a high-throughput clinical assay for detecting antibodies that neutralize SARS-CoV-2 [bioRxiv, 2020-05-27]

We evaluated a lateral flow immunoassay (AllTest COVID-19 IgG/IgM) which detects IgG and IgM antibodies.

... was to analyze the diagnostic performance of one serologic rapid test in COVID-19 patients. METHODS: We evaluated a lateral flow immunoassay (AllTest COVID-19 IgG/IgM) which detects IgG and IgM antibodies. We validated the serologic test using serum samples from 100 negative patients (group 1) and ...

Ref: Alltest rapid lateral flow immunoassays is reliable in diagnosing SARS-CoV-2 infection from 14 days after symptom onset: A prospective single-center study [J Clin Virol, 2020]

Among the positive samples, 11 had SARS-CoV-2 neutralizing antibodies with a titer ranging from 1/20 to 1/1080.

... for the receptor binding domain (RBD) of SARS-CoV-2 by indirect enzyme linked immunosorbent assay (ELISA). Among the positive samples, 11 had SARS-CoV-2 neutralizing antibodies with a titer ranging from 1/20 to 1/1080. No serological cross-reactivity was detected between the SARS-CoV-2 and type I or II feline infectious ...

Ref: SARS-CoV-2 neutralizing serum antibodies in cats: a serological investigation [bioRxiv, 2020-04-03]

Here, we evaluated four high throughput serologic tests for detection of anti-SARS-CoV-2 IgG antibodies,

... continue to evolve as we gain increasing insight into our immune response to the virus. Here, we evaluated four high throughput serologic tests for detection of anti-SARS-CoV-2 IgG antibodies, including assays from Abbott Laboratories (Abbott Park, IL), Epitope Diagnostics Inc. (San Diego, CA), Euroimmun ...

Ref: Performance Characteristics of Four High-Throughput Immunoassays for Detection of IgG Antibodies against SARS-CoV-2 [J. clin. microbiol, 2020]

Serological assay was performed using an Enzyme-Linked Immunosorbent Assays kit (Lizhu, Zhuhai, China),

... Serological assay was performed using an Enzyme-Linked Immunosorbent Assays kit (Lizhu, Zhuhai, China), which was developed for detecting IgM or IgG antibody against N proteins of SARS-CoV-2. For IgM detection, ELISA plates were previously coated with mouse anti-human IgM (m chain) monoclonal antibody. ...

Ref: A preliminary study on serological assay for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in 238 admitted hospital patients [Microbes Infect, 2020-05-18]

Fully automated CLIA and ELISA assays allow the quantitative determination of antibodies against SARS-CoV-2 by clinical laboratories with increased screening capacity.

... Fully automated CLIA and ELISA assays allow the quantitative determination of antibodies against SARS-CoV-2 by clinical laboratories with increased screening capacity. In this study, the ELISA IgG/IgA was tested on a fully automated microtiter plate analyser. The workflow for ELISA tests depends on the analyser used: in our laboratory, the capacity ...

Ref: Evaluation of two automated and three rapid lateral flow immunoassays for the detection of anti-SARS-CoV-2 antibodies [J Clin Virol, 2020-05-05]

Confirmed SARS-CoV-2 positive-serum 119 and negative-serum were used as controls in each set test.

... as relative light 118 units, and the whole testing can be completed in 23 minutes. Confirmed SARS-CoV-2 positive-serum 119 and negative-serum were used as controls in each set test. square and Fisher"s exact test was used for comparing the difference between the analyzed groups. ...

Ref: Evaluations of serological test in the diagnosis of 2019 novel coronavirus (SARS-CoV-2) infections during the COVID-19 outbreak [J Clin Virol, 2020-03-30]

Current serological tests developed for SARS-CoV-2 rely on traditional technologies such as enzyme-linked immunosorbent assays (ELISA) and lateral flow assays,

... prevalence of infection, as well as ascertain individuals who may be protected from future infection. Current serological tests developed for SARS-CoV-2 rely on traditional technologies such as enzyme-linked immunosorbent assays (ELISA) and lateral flow assays, which may lack scalability to meet the demand of hundreds of millions of antibody tests ...

Ref: A rapid, point of care red blood cell agglutination assay for detecting antibodies against SARS-CoV-2 [bioRxiv, 2020-05-13]

Numerous serologic immunoassays have been launched to detect antibodies to SARS-CoV-2, including rapid tests.

... Objectives: Numerous serologic immunoassays have been launched to detect antibodies to SARS-CoV-2, including rapid tests. Here, we validate use of a lateral flow immunoassay (LFI) intended for rapid screening and qualitative detection of anti-SARS-CoV-2 IgM and IgG in serum, plasma, and whole blood, and ...

Ref: Analytical performance of lateral flow immunoassay for SARS-CoV-2 exposure screening on venous and capillary blood samples [bioRxiv, 2020-05-18]

The absence of FDA oversight of serologic tests is concerning, given that the commercially available serologic assays are highly variable,

... molecular tests, submission for EUA is currently a voluntary process for manufacturers of serologic assays. The absence of FDA oversight of serologic tests is concerning, given that the commercially available serologic assays are highly variable, differing in their format, the antibody class detected, the targeted antigen and the acceptable specimen ...

Ref: The Role of Antibody Testing for SARS-CoV-2: Is There One? [Journal of clinical microbiology, 2020-04-29]

A total of 535 plasma samples collected during the hospitalization period of the 173 patients were tested for antibodies against SARS-CoV-2.

... A total of 535 plasma samples collected during the hospitalization period of the 173 patients were tested for antibodies against SARS-CoV-2. The seroconversion rate for Ab, IgM and IgG was 93.1% (161/173), 82.7% (143/173) and 64.7% (112/173), respectively (Table 1) . ...

Ref: Antibody responses to SARS-CoV-2 in patients of novel coronavirus disease 2019 [Clin Infect Dis, 2020-03-28]

Multiple serologic tests have been developed for COVID-19 5 including a recently FDA-approved lateral flow assay.

... PCR positivity, SARS-CoV-2 antibodies are detectable throughout the disease course and persist indefinitely 4 . Multiple serologic tests have been developed for COVID-19 5 including a recently FDA-approved lateral flow assay. However, these tests are limited to detection of antibodies against one or two antigens, and ...

Ref: Analysis of SARS-CoV-2 Antibodies in COVID-19 Convalescent Plasma using a Coronavirus Antigen Microarray [bioRxiv, 2020-04-17]

Serological testing is a complementary approach that detects the presence of antibodies against SARS-CoV-2 in blood samples from exposed individuals 2 .

... Serological testing is a complementary approach that detects the presence of antibodies against SARS-CoV-2 in blood samples from exposed individuals 2 . Antibodies specific to viral proteins will emerge 3-10 days after infection, with IgM appearing first (transiently) and IgG becoming detectable approximately 14 days after infection 3 . IgG antibodies remain ...

Ref: Enzyme immunoassay for SARS-CoV-2 antibodies in dried blood spot samples: A minimally-invasive approach to facilitate community- and population-based screening [bioRxiv, 2020-05-04]

10 Serological assays can take many forms, including enzyme-linked immunosorbent assays (ELISA) 11 , viral neutralization assays, and rapid lateral flow assays.

... for monitoring how a patient"s anti-viral response evolves after the period of acute infection ends. 10 Serological assays can take many forms, including enzyme-linked immunosorbent assays (ELISA) 11 , viral neutralization assays, and rapid lateral flow assays. 10 Neutralization assays with serum necessitate culture of either live or pseudovirus, and the market ...

Ref: A SARS-CoV-2 serological assay to determine the presence of blocking antibodies that compete for human ACE2 binding [medRxiv, 2020-05-29]

In ELISA-based serological tests, fluorescence is determined in units of optical density or 'arbitrary units'.

... in controls and conformed cases. Such quantitative measures differ depending on the diagnostic techniques used. In ELISA-based serological tests, fluorescence is determined in units of optical density or "arbitrary units". Tests involving neutralization assays usually report the serum titers needed to achieve viral neutralization of ...

Ref: Estimating seroprevalence with imperfect serological tests: exploiting cutoff-free approaches [bioRxiv, 2020-05-19]

Focusing on IgG antibodies, we demonstrate the performance of two 28 ELISA assays

... tests perform with real patient samples and if detected IgG antibodies 27 provide protective immunity. Focusing on IgG antibodies, we demonstrate the performance of two 28 ELISA assays (Euroimmun SARS-CoV-2 IgG & Vircell COVID-19 ELISA IgG) in comparison to one lateral 29 flow ...

Ref: Clinical performance of SARS-CoV-2 IgG antibody tests and potential protective immunity [bioRxiv, 2020-05-10]

serological methods can also be used to detect specific antibodies of the IgM, IgG and IgA class produced after contact with antigens or to detect viral antigen.

... case definition. However, in the laboratory diagnosis of SARS-CoV-2 infections, in addition to genetic tests, serological methods can also be used to detect specific antibodies of the IgM, IgG and IgA class produced after contact with antigens or to detect viral antigen. Currently, a number of rapid immunochromatographic, chemiluminescent and ELISA immunoassay tests developed by different manufacturers ...

Ref: Characteristics and assessment of the usefulness of serological tests in the diagnostic of infections caused by coronavirus SARS-CoV-2 on the basis of available manufacturer's data and literature review. [Przeglad epidemiologiczny, 2020]

Conventional serological assays, such as enzyme-linked immunoassay (ELISA) for specific IgM and IgG antibodies,

... as delayed diagnosis. Furthermore, there has not been enough time to assess specificity and sensitivity. Conventional serological assays, such as enzyme-linked immunoassay (ELISA) for specific IgM and IgG antibodies, should offer a high-throughput alternative, which allows for uniform tests for all suspected patients, and ...

Ref: Evolving status of the 2019 novel coronavirus infection: Proposal of conventional serologic assays for disease diagnosis and infection monitoring [J Med Virol, 2020-02-17]

Methods A newly-developed ELISA assay for IgM and IgG antibodies against N protein of SARS-CoV-2

... public health laboratories. We aimed to evaluate the diagnostic value of serological assay for SARS-CoV-2. Methods A newly-developed ELISA assay for IgM and IgG antibodies against N protein of SARS-CoV-2 were used to screen the serums of 238 admitted hospital patients with confirmed or suspected ...

Ref: A preliminary study on serological assay for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in 238 admitted hospital patients [J Med Virol, 2020-03-08]

Serological tests detect antibodies against the suspected pathogen,

... relating to the suspected pathogen is indicative of an active infection with the suspected pathogen. Serological tests detect antibodies against the suspected pathogen, which are produced by an individual"s immune system. A positive serological test result indicates recent ...

Ref: COVID-19 Special Column: Principles Behind the Technology for Detecting SARS-CoV-2, the Cause of COVID-19 [Hawaii J Health Soc Welf, 2020]

We evaluated specificity and sensitivity of six commercial immunoassays for the detection of SARS-CoV-2 IgG, IgA and IgM antibodies,

... novel virus should be determined with clinical specimens against a gold standard, i.e. virus neutralisation. We evaluated specificity and sensitivity of six commercial immunoassays for the detection of SARS-CoV-2 IgG, IgA and IgM antibodies, including four automated assays [Abbott SARS-COV-2 IgG (CE marked), Diasorin Liaison SARS-CoV-2 S1/S2 IgG (research ...

Ref: Performance of six SARS-CoV-2 immunoassays in comparison with microneutralisation [Hawaii J Health Soc Welf, 2020-05-22]

Existing diagnostic assays for SARS-CoV can detect antibodies in patients who were diagnosed with COVID-19.

... test was not useful in the early phase of the illness with poor sensitivity. Conclusions: Existing diagnostic assays for SARS-CoV can detect antibodies in patients who were diagnosed with COVID-19. These assays maybe be utilized as an interim measure in epidemiological investigations for contact tracing ...

Ref: Cross-reaction of sera from COVID-19 patients with SARS-CoV assays. [Hawaii J Health Soc Welf, 2020-03-23]

the RBD represents a promising antigen for detecting CoV-specific antibodies in people.

... (RBD) of the spike protein is poorly conserved between SARS-CoVs and other pathogenic human coronaviruses, the RBD represents a promising antigen for detecting CoV-specific antibodies in people. Here we use a large panel of human sera (63 SARS-CoV-2 patients and 71 control ...

Ref: The receptor binding domain of the viral spike protein is an immunodominant and highly specific target of antibodies in SARS-CoV-2 patients. [Science immunology, 2020-06-11]

Weak signals for IgM and IgG, together or separate, were considered positive.

... The copyright holder for this preprint this version posted June 17, 2020. were read visually after 10 min. Weak signals for IgM and IgG, together or separate, were considered positive. All tests were conducted at room temperature according to each manufacturer"s instruction. ...

Ref: Seroprevalence of Antibodies to SARS-CoV-2 in Healthcare Workers in Non-epidemic Region: A Report from Iwate Prefecture in Japan [Science immunology, 2020-06-17]

Cross reactivity with non-SARS-CoV-2 coronaviruses by the four POC rapid antibody tests was not well investigated (Table 1) .

... study. Therefore, we could not exclude the possibility of co-infection in our patients with COVID-19. Cross reactivity with non-SARS-CoV-2 coronaviruses by the four POC rapid antibody tests was not well investigated (Table 1) . Fifth, pneumonia diagnosis of the COVID-19 patients was primarily based on chest roentgenogram in this ...

Ref: Four point-of-care lateral flow immunoassays for diagnosis of COVID-19 and for assessing dynamics of antibody responses to SARS-CoV-2 [J Infect, 2020-06-15]

Serological tests that can detect SARS-CoV-2 IgG-IgM antibodies are simpler 248 than rRT-PCR,

... leading to the administration of appropriate antimicrobial agents. Serological tests that can detect SARS-CoV-2 IgG-IgM antibodies are simpler 248 than rRT-PCR, and do not require complicated equipment and protocols 249 (Table 3) . Thus, these tests can be used for rapid detection and ...

Ref: In vitro diagnostics of coronavirus disease 2019: technologies and application [J Microbiol Immunol Infect, 2020-06-05]

Serological assays are urgently needed to conduct serosurveys, to understand the antibody responses mounted in response to the virus,

... allow detection of the virus during a short period of time, generally during acute infection. Serological assays are urgently needed to conduct serosurveys, to understand the antibody responses mounted in response to the virus, and to identify individuals who are potentially immune to re-infection. Here we describe a detailed ...

Ref: SARS-CoV-2 Seroconversion in Humans: A Detailed Protocol for a Serological Assay, Antigen Production, and Test Setup [Curr Protoc Microbiol, 2020]

Numerous antibody tests have recently become available.

... Numerous antibody tests have recently become available. Serologic tests for antibodies to SARS-CoV-2 are typically based on lateral flow immunochromatography or enzyme-linked immunosorbent assays (ELISA). Currently available tests predominantly target antibodies to 1 of 2 main surface ...

Ref: Double-Edged Spike: Are SARS-CoV-2 Serologic Tests Safe Right Now? [Am J Clin Pathol, 2020-04-23]

Serology testing seems to have a higher sensitivity than molecular diagnostics from 8 days after onset of symtoms,

... coronavirus 2 (sars-cov-2) after covid-19, but it remains unclear for how long the protection remains. Serology testing seems to have a higher sensitivity than molecular diagnostics from 8 days after onset of symtoms, and should be part of risk assessment and epidemiological studies of COVID-19. The performance of ...

Ref: [Rapid point-of-care serology testing for sars-cov-2]. [Lakartidningen, 2020-05-27]

Antibodies against SARS-CoV-2 were detected in 56 out of 63 patients (88.9%),

... We assessed the serologic test in the group 3 patients. Antibodies against SARS-CoV-2 were detected in 56 out of 63 patients (88.9%), being 100% of them positive for IgG antibodies, and 39.7% positive also for IgM antibodies (Table 1) . No patient ...

Ref: Rapid diagnosis of SARS-CoV-2 infection by detecting IgG and IgM antibodies with an immunochromatographic device: a prospective single-center study [Lakartidningen, 2020-04-15]

Lately, several commercial assays for the determination of SARS-CoV-2 IgG became available.

Lately, several commercial assays for the determination of SARS-CoV-2 IgG became available. In this study, we compared four assays in respect to their sensitivity and specificity.

Ref: Comparison of four new commercial serologic assays for determination of SARS-CoV-2 IgG [J Clin Virol, 2020-04-29]

The gold-standard test is ELISA (enzyme-linked immunosorbent assay).

... begins at a mean 10-20 days after onset of infection ( Fig. 1) [1] . The gold-standard test is ELISA (enzyme-linked immunosorbent assay). Performed in the laboratory on venous blood, this colorimetric reaction includes quantitative spectrometry, specifying IgG ...

Ref: Keys for analysis of diagnostic and serologic tests for CoV-2 [Eur Ann Otorhinolaryngol Head Neck Dis, 2020-05-27]

the Abbott SARS-CoV-2 IgG test has high technical specificity.

... antibodies confer long-term immunity in humans. This is vital for several reasons including In conclusion, the Abbott SARS-CoV-2 IgG test has high technical specificity. There is 195 potential room for improving clinical sensitivity of the assay to detect previous ...

Ref: Clinical evaluation of serological IgG antibody response on the Abbott Architect for established SARS-CoV-2 infection [Clin Microbiol Infect, 2020-06-09]

Serological platforms were developed and assessed for confirmation of SARSCoV2specific antibody responses to capture past infections.

... of severe acute respiratory syndrome coronavirus 2 (SARSCoV2) by one individual interacting with both clusters. Serological platforms were developed and assessed for confirmation of SARSCoV2specific antibody responses to capture past infections. By serological analysis, Yong and colleagues identified the missing link between the Church A cluster ...

Ref: The important role of serology for COVID-19 control [Lancet Infect Dis, 2020-04-21]

Appropriate quality assurance measures for molecular and serological assays are needed to maintain testing proficiency.

... the design of primers, the selection of specimens and testing strategies at different disease stages. Appropriate quality assurance measures for molecular and serological assays are needed to maintain testing proficiency. Because serological assays have the potential to identify later stages of the infection and to ...

Ref: Molecular and serological assays for SARS-CoV-2: insights from genome and clinical characteristics [Clin. chem, 2020]

We observed a strong correlation between levels of RBD binding antibodies and SARS-CoV-2 neutralizing antibodies in patients.

... SARS-CoV-2 RBD antigen was highly sensitive (98%) and specific (100%) for antibodies induced by SARS-CoVs. We observed a strong correlation between levels of RBD binding antibodies and SARS-CoV-2 neutralizing antibodies in patients. Our results, which reveal the early kinetics of SARS-CoV-2 antibody responses, support using the RBD ...

Ref: The receptor binding domain of the viral spike protein is an immunodominant and highly specific target of antibodies in SARS-CoV-2 patients. [Science immunology, 2020-06-11]

We evaluated 4 immunochromatographic tests for the detection of IgM and IgG directed against SARS-CoV-2 ( Figure 1 ).

... We evaluated 4 immunochromatographic tests for the detection of IgM and IgG directed against SARS-CoV-2 ( Figure 1 ). These tests were kindely provided by Asian manufacturers, namely Biotime Biotechnology Co, Autobio Diagnostics Co, ISIA BIO-Technology Co and Biolidics. ...

Ref: Dynamic profile for the detection of anti-SARS-CoV-2 antibodies using four immunochromatographic assays [J Infect, 2020-05-07]

rapid lateral flow assays for antibodies (IgM and IgG) produced during COVID-19 have been developed (70) .

... As mentioned above, rapid lateral flow assays for antibodies (IgM and IgG) produced during COVID-19 have been developed (70) . Seroconversion occurred after 7 days of symptomatic infection in 50% of patients (14 days in all) but was not followed by a rapid decline in viral load ...

Ref: Laboratory Diagnosis of COVID-19: Current Issues and Challenges [J Clin Microbiol, 2020-05-26]

Serum samples were tested for specific IgM, IgA and IgG using 1:20 screening dilution.

... prepared in-house with Vero E6 cells infected with SARS-CoV-2 isolate (2019-nCoV/Italy-INMI1), as described elsewhere (5). Serum samples were tested for specific IgM, IgA and IgG using 1:20 screening dilution. FITC-conjugated anti-human IgM, IgA and IgG antibodies (Euroimmun, Germany) were used as secondary antibody and ...

Ref: Evaluation of ELISA tests for the qualitative determination of IgG, IgM and IgA to SARS-CoV-2 [J Clin Microbiol, 2020-05-26]

several serological tests, including the enzyme-linked immunosorbent assay (ELISA), the chemiluminescence immunoassay (CLIA) and lateral flow rapid testing,

... for research on in-vitro diagnostics for use at the community level [4] . In response, several serological tests, including the enzyme-linked immunosorbent assay (ELISA), the chemiluminescence immunoassay (CLIA) and lateral flow rapid testing, are now under development or have already entered the market. The Foundation for Innovative New ...

Ref: Evaluation of two automated and three rapid lateral flow immunoassays for the detection of anti-SARS-CoV-2 antibodies [J Clin Virol, 2020-05-05]

Here, we describe a serological enzyme-linked immunosorbent assay for the screening and identification of human SARS-CoV-2 seroconverters.

... Here, we describe a serological enzyme-linked immunosorbent assay for the screening and identification of human SARS-CoV-2 seroconverters. This assay does not require the handling of infectious virus, can be adjusted to detect different antibody types in serum and plasma and is amenable to scaling. Serological assays are ...

Ref: A serological assay to detect SARS-CoV-2 seroconversion in humans. [Nature medicine, 2020-05-12]

Alltest COVID-19 IgG/IgM lateral flow immunoassay is reliable to diagnose SARS-CoV-2 infection

Alltest COVID-19 IgG/IgM lateral flow immunoassay is reliable to diagnose SARS-CoV-2 infection as a complement of PCR from 14 days after the onset of symptoms.

Ref: Alltest rapid lateral flow immunoassays is reliable in diagnosing SARS-CoV-2 infection from 14 days after symptom onset: a prospective single-center study [J Clin Virol, 2020-05-27]

The EUROIMMUN Anti-SARS-CoV-2 ELISA Assay demonstrates good sensitivity for detection of IgA and excellent sensitivity for detection of IgG antibodies

... for IgA, and 42 of 42 (100% 95% CI: 91.6–100) were positive for IgG, respectively. The EUROIMMUN Anti-SARS-CoV-2 ELISA Assay demonstrates good sensitivity for detection of IgA and excellent sensitivity for detection of IgG antibodies from samples collected ≥4 days, after COVID-19 diagnosis by PCR. This assay demonstrated only borderline ...

Ref: Evaluation of the EUROIMMUN Anti-SARS-CoV-2 ELISA Assay for detection of IgA and IgG antibodies [J Clin Virol, 2020-05-23]

We developed a luminescent immunoassay for IgG and IgM against SARS-CoV-2,

... We developed a luminescent immunoassay for IgG and IgM against SARS-CoV-2, which, to our knowledge, was the first such assay allowing us to study the antibody response to the newly identified coronavirus. This assay was based on a peptide from the ...

Ref: A Peptide-based Magnetic Chemiluminescence Enzyme Immunoassay for Serological Diagnosis of Coronavirus Disease 2019 (COVID-19) [J Infect Dis, 2020-05-08]

enzyme-linked immunosorbent assay and chemiluminescence immunoassay (CLIA),

... also dangerous making these tests potentially less reliable. Along with chromatographic rapid immunoassays as qualitative tests, 10 quantitative antibodies detection tests, such as enzyme-linked immunosorbent assay and chemiluminescence immunoassay (CLIA), have spread often by fully automated analyzers. ...

Ref: Diagnostic accuracy of an automated chemiluminescent immunoassay for anti‐SARS‐CoV‐2 IgM and IgG antibodies: an Italian experience [J Med Virol, 2020-05-10]

Various techniques can be used to detect specific antibodies directed against flaviviruses.

... that are used in routine practice and those that are commercially available for flavivirus serology. Various techniques can be used to detect specific antibodies directed against flaviviruses. For serum specimens, hemagglutination inhibition assay and complement fixation techniques have been progressively replaced by ...

Ref: Serological Diagnosis of Flavivirus-Associated Human Infections [Diagnostics (Basel), 2020-05-14]

Cross-reactivity with serum samples from HCoV 243 patients has been shown for serological SARS-CoV-2 IgA and IgG antibody assays 4 .

... decades and led to the earlier categorization of 242 corona viruses into serogroups 31 . Cross-reactivity with serum samples from HCoV 243 patients has been shown for serological SARS-CoV-2 IgA and IgG antibody assays 4 . 244 Therefore, in order to make a valid serological diagnosis of SARS-CoV-2 neutralizing 245 antibodies, ...

Ref: SARS-CoV-2 Antibody Testing – Questions to be asked [J Allergy Clin Immunol, 2020-05-29]

Although detection of antibodies to measure exposure, immunity, or both seems straightforward conceptually,

... influenza pandemics) has made relevant contributions, but specific conditions of the current situation require adaptation. Although detection of antibodies to measure exposure, immunity, or both seems straightforward conceptually, numerous challenges exist in terms of sample collection, what the presence of antibodies actually means, ...

Ref: Seroepidemiologic Study Designs for Determining SARS-COV-2 Transmission and Immunity. [Emerging infectious diseases, 2020-06-16]

Abbott SARS-CoV-2 IgG test for detection of anti-SARS-CoV-2 IgG antibodies by testing 3 distinct patient populations.

... 2020, necessitating the use of serological testing to determine past infections. Here, we evaluated the Abbott SARS-CoV-2 IgG test for detection of anti-SARS-CoV-2 IgG antibodies by testing 3 distinct patient populations. We tested 1,020 serum specimens collected prior to SARS-CoV-2 circulation in the United States and ...

Ref: Performance Characteristics of the Abbott Architect SARS-CoV-2 IgG Assay and Seroprevalence Testing in Idaho [Emerging infectious diseases, 2020-05-02]

We evaluated precision, analytical interferences, and cross-reactivity with other viral infections.

... performed validation studies according to COLA guidelines, using samples previously tested for SARS-CoV-2 by RT-PCR. We evaluated precision, analytical interferences, and cross-reactivity with other viral infections. We also evaluated concordance with molecular and other serological testing, and evaluated seroconversion. Results: The ...

Ref: Clinical validation and performance evaluation of the automated Vitros Total Anti-SARS-CoV-2 Antibodies assay for screening of serostatus in COVID-19 [Emerging infectious diseases, 2020-06-12]

A wide range of serology immunoassays (IAs) have been developed as well [7] [8] .

... although evidence arose that its accuracy could be not maximum [3] [4] [5] [6] . A wide range of serology immunoassays (IAs) have been developed as well [7] [8] . These include automated chemiluminescent IA (CLIA), manual ELISA, and rapid lateral flow IA (LFIA), which ...

Ref: Evaluating COVID-19 screening strategies based on serological tests [Emerging infectious diseases, 2020-06-14]

SARS-CoV-2 specific antibodies are usually only detected more than one week after onset of symptoms [5] ,

SARS-CoV-2 specific antibodies are usually only detected more than one week after onset of symptoms [5] , limiting the role of serology for identification of acute infection.

Ref: Comparison of four new commercial serologic assays for determination of SARS-CoV-2 IgG [J Clin Virol, 2020-04-29]

The LIAISON® SARS-CoV-2 S1/S2 IgG test detects IgG-antibodies against the S1/S2 domains of

The copyright holder for this preprint this version posted June 5, 2020. 3. The LIAISON® SARS-CoV-2 S1/S2 IgG test detects IgG-antibodies against the S1/S2 domains of

Ref: Side by side comparison of three fully automated SARS-CoV-2 antibody assays with a focus on specificity [J Clin Virol, 2020-06-05]

Here, we evaluated the Abbott SARS-CoV-2 IgG test for detection of anti-SARS-CoV-2 IgG antibodies

... in February and March 2020, necessitating the use of serological testing to determine past infections. Here, we evaluated the Abbott SARS-CoV-2 IgG test for detection of anti-SARS-CoV-2 IgG antibodies by testing 3 distinct patient populations. We tested 1,020 serum specimens collected prior to SARS-CoV-2 ...

Ref: Performance Characteristics of the Abbott Architect SARS-CoV-2 IgG Assay and Seroprevalence Testing in Idaho [J Clin Virol, 2020-05-02]

We performed validation studies according to COLA guidelines, using samples previously tested for SARS-CoV-2 by RT-PCR.

... of the Vitros Anti-SARS-CoV-2 Total (CoV2T) assay for qualitative serological testing of SARS-CoV-2 antibodies. Methods: We performed validation studies according to COLA guidelines, using samples previously tested for SARS-CoV-2 by RT-PCR. We evaluated precision, analytical interferences, and cross-reactivity with other viral infections. We also evaluated concordance ...

Ref: Clinical validation and performance evaluation of the automated Vitros Total Anti-SARS-CoV-2 Antibodies assay for screening of serostatus in COVID-19 [J Clin Virol, 2020-06-12]

After the incubation, the cells were fixed and stained with polyclonal rabbit anti-SARS-CoV antibody (Sino Biological).

... more hour. The cells were then washed and further incubated in medium for 8 h. After the incubation, the cells were fixed and stained with polyclonal rabbit anti-SARS-CoV antibody (Sino Biological). The cells were then fixed and stained using a rabbit anti-SARS-CoV serum and a secondary ...

Ref: SARS-CoV-2 specific antibody responses in COVID-19 patients [J Clin Virol, 2020-03-20]

Case serum samples (n = 128) were obtained from COVID-19 patients confirmed by RT-qPCR and CT-scan.

... tests were performed. This retrospective study included 200 residual sera from patients and healthy volunteers. Case serum samples (n = 128) were obtained from COVID-19 patients confirmed by RT-qPCR and CT-scan. Days since onset of symptoms was collected from their medical records. Control non-SARS-CoV-2 samples (n ...

Ref: Evaluation of two automated and three rapid lateral flow immunoassays for the detection of anti-SARS-CoV-2 antibodies [J Clin Virol, 2020]

immunochromatographic lateral flow assays (LFAs) and enzyme-linked immunosorbent assays (ELISAs).

... https://doi.org/10.1101 We conducted a head-to-head comparison of serology tests available to our group -comprised of immunochromatographic lateral flow assays (LFAs) and enzyme-linked immunosorbent assays (ELISAs). Our evaluation includes performance by time from symptom onset and disease severity. Our goal is ...

Ref: Test performance evaluation of SARS-CoV-2 serological assays [medRxiv, 2020-05-17]

SARS-CoV-2 IgM and IgG antibodies can be quantified with enzyme linked immunosorbent assay (ELISA),

... from reacquisition of COVID-19 or how to roll out vaccine access to those still susceptible. SARS-CoV-2 IgM and IgG antibodies can be quantified with enzyme linked immunosorbent assay (ELISA), and protocols for serum or plasma samples are now established 6 . However, the use ...

Ref: Enzyme immunoassay for SARS-CoV-2 antibodies in dried blood spot samples: A minimally-invasive approach to facilitate community- and population-based screening [medRxiv, 2020-05-04]

10 quantitative antibodies detection tests, such as enzyme-linked immunosorbent assay and chemiluminescence immunoassay (CLIA),

... of control in the production process is also dangerous making these tests potentially less reliable. Along with chromatographic rapid immunoassays as qualitative tests, 10 quantitative antibodies detection tests, such as enzyme-linked immunosorbent assay and chemiluminescence immunoassay (CLIA), have spread often by fully automated analyzers. ...

Ref: Diagnostic accuracy of an automated chemiluminescent immunoassay for anti‐SARS‐CoV‐2 IgM and IgG antibodies: an Italian experience [J Med Virol, 2020-05-10]