¿Cuál es la estructura proteica del peplómero del SARS-CoV-2?
(What is the protein structure of the sars-cov-2 spike?)
Primeras 5 respuestas:
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The spike protein on the coronavirus envelope is a trimeric protein.
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The spike (S) glycoprotein of SARS-CoV-2 is responsible for the binding to the permissive cells.
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One of such proteins is the SARS-CoV-2 main protease M pro ,
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Protein-protein interaction structure modeling indicates that spike-RBD of the two viruses also has similar overall binding conformation and binding free energy to ACE2.
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The three-dimensional structure of SARS-CoV-2 Spike protein
The spike protein on the coronavirus envelope is a trimeric protein.
... The spike protein on the coronavirus envelope is a trimeric protein. This protein is critical for the vitality of coronaviruses because it is not only an important component for the virus particle but also plays a crucial role in attaching host ...
Ref: Molecular Mechanism of Evolution and Human Infection with SARS-CoV-2 [Viruses, 2020-04-10]The spike (S) glycoprotein of SARS-CoV-2 is responsible for the binding to the permissive cells.
... The spike (S) glycoprotein of SARS-CoV-2 is responsible for the binding to the permissive cells. The receptor-binding domain (RBD) of SARS-CoV-2 S protein directly interacts with the human angiotensin-converting enzyme 2 (ACE2) on the host cell membrane. In this study, we used computational saturation mutagenesis ...
Ref: Systemic Effects of Missense Mutations on SARS-CoV-2 Spike Glycoprotein Stability and Receptor Binding Affinity [bioRxiv, 2020-05-23]One of such proteins is the SARS-CoV-2 main protease M pro ,
... life cycle proteins that also serve as attractive targets for the development of anti-SARS-CoV-2 therapeutics. One of such proteins is the SARS-CoV-2 main protease M pro , which is required for processing the polyproteins translated from the viral RNA. Inhibition of the ...
Ref: Molecular structure analyses suggest strategies to therapeutically target SARS-CoV-2 [Nat Commun, 2020-06-10]Protein-protein interaction structure modeling indicates that spike-RBD of the two viruses also has similar overall binding conformation and binding free energy to ACE2.
... SARS-CoV-2 and SARS-CoV share a common human receptor ACE2. Protein-protein interaction structure modeling indicates that spike-RBD of the two viruses also has similar overall binding conformation and binding free energy to ACE2. In vitro assays using recombinant ACE2 proteins and ACE2 expressing cells confirmed the two coronaviruses’ similar binding affinities to ACE2. The ...
Ref: SARS-CoV-2 and SARS-CoV Spike-RBD Structure and Receptor Binding Comparison and Potential Implications on Neutralizing Antibody and Vaccine Development [bioRxiv, 2020-02-20]The three-dimensional structure of SARS-CoV-2 Spike protein
... The three-dimensional structure of SARS-CoV-2 Spike protein ( Figure The Ramachandran scores of the modeled ACE2 structures for selected species are summarized in Table 1 with the actual predicted ACE2 structures and Ramachandran plots for each selected ...
Ref: In silico comparison of spike protein-ACE2 binding affinities across species; significance for the possible origin of the SARS-CoV-2 virus [bioRxiv, 2020-05-13]Spike (S) glycoprotein is the structural protein of SARS-CoV-2 located on the envelope surface,
... is continued to spread globally and no potential drug or vaccine against it is available. Spike (S) glycoprotein is the structural protein of SARS-CoV-2 located on the envelope surface, involve in interaction with angiotensin converting enzyme 2 (ACE2), a cell surface receptor, followed by ...
Ref: Truncated human angiotensin converting enzyme 2; a potential inhibitor of SARS-CoV-2 spike glycoprotein and potent COVID-19 therapeutic agent [J Biomol Struct Dyn, 2020-05-20]The four structural proteins include spike, envelope, membrane, and nucleocapsid proteins,
... of a large RNA genome, four structural proteins, 16 nonstructural proteins, and some accessory proteins. The four structural proteins include spike, envelope, membrane, and nucleocapsid proteins, of which the spike glycoprotein is of particular interest for it is a popular vaccine ...
Ref: Human H-ferritin presenting RBM of spike glycoprotein as potential vaccine of SARS-CoV-2 [bioRxiv, 2020-06-08]Human ACE2 is now established as a receptor for the SARS-CoV-2 spike protein.
... the most fearsome compared with the earlier outbreaks caused by other coronaviruses, SARS-CoV and MERS-CoV. Human ACE2 is now established as a receptor for the SARS-CoV-2 spike protein. Where variations in the viral spike protein, in turn, lead to the cross-species transmission of ...
Ref: Structural variations in human ACE2 may influence its binding with SARS-CoV-2 spike protein [J. med. virol, 2020]SARS-CoV-2 encodes at least 27 proteins, including 15 nonstructural proteins, 4 structural proteins, and 8 auxiliary proteins.
... SARS-CoV-2 encodes at least 27 proteins, including 15 nonstructural proteins, 4 structural proteins, and 8 auxiliary proteins. 12 Spike glycoprotein (S), a structural protein located on the outer envelope of the virion, binds to the host-receptor angiotensin-converting enzyme 2 (ACE2). The S glycoprotein of SARS-CoV, MERS-CoV, and ...
Ref: Composition and divergence of coronavirus spike proteins and host ACE2 receptors predict potential intermediate hosts of SARS‐CoV‐2 [J Med Virol, 2020-03-11]Spike protein of SARS-CoV-2 is 1273 amino acid long protein with two functionally distinct regions,
... 4 (DPP4) as the first site of attachment to the host cell (Li, 2015) . Spike protein of SARS-CoV-2 is 1273 amino acid long protein with two functionally distinct regions, S1 and S2, involved in the attachment and entry of the virus, respectively. SARS-CoV-2 entry ...
Ref: Structural Basis of SARS-CoV-2 Spike Protein Priming by TMPRSS2 [bioRxiv, 2020-04-21]The Spike protein is a large type I transmembrane protein of approximately 1400 amino acids.
... The Spike protein is a large type I transmembrane protein of approximately 1400 amino acids. S protein is an attractive target for vaccine development, as its surface expression renders it a direct target for the host immune response. Spike proteins assemble into trimers on the ...
Ref: Identification of conserved epitopes in SARS-CoV-2 spike and nucleocapsid protein [bioRxiv, 2020-05-14]SARS-CoV-2 spike glycoprotein is a 1273 amino-acid-long structural protein located on the outer envelope of the virus.
... SARS-CoV-2 spike glycoprotein is a 1273 amino-acid-long structural protein located on the outer envelope of the virus. The protein has two main functional subunits: a long N-terminal S1 subunit and a relatively short C-terminal S2 subunit. A 200 amino-acid-long RBD is stationed within the S1 subunit of ...
Ref: Structural variations in human ACE2 may influence its binding with SARS‐CoV‐2 spike protein [J Med Virol, 2020-04-15]The spike protein protrudes from the envelope of the virion and consists of two subunits;
... coronavirus particles contain four main structural proteins: the spike, membrane, envelope, and nucleocapsid. [1] [2] The spike protein protrudes from the envelope of the virion and consists of two subunits; a receptor-binding domain (RBD) that interacts with the receptor proteins of host cells and a ...
Ref: Impact of Thiol-Disulfide Balance on the Binding of Covid-19 Spike Protein with Angiotensin Converting Enzyme 2 Receptor [bioRxiv, 2020-05-11]The spike, nucleocapsid, membrane, and envelope proteins are structural proteins.
... positive viral RNA genome expressing open reading frames that code for structural and non-structural proteins. The spike, nucleocapsid, membrane, and envelope proteins are structural proteins. The S1 subunit of spike protein facilitates ACE2 mediated virus attachment and S2 subunit for ...
Ref: Structural Proteins in Severe Acute Respiratory Syndrome Coronavirus-2 [Arch Med Res, 2020-05-25]The viral particles contain four main structural proteins: the spike, membrane, envelope protein, and nucleocapsid.
... epi-demiological outbreaks. These are enveloped viruses with a positive-sense single-strand RNA of around 32 Kb. The viral particles contain four main structural proteins: the spike, membrane, envelope protein, and nucleocapsid. The spike protein protrudes from the envelope of the virion and plays a pivotal role ...
Ref: cord_uid ldf7uso2 Role of changes in SARS-CoV-2 spike protein in... ldf7uso2 Role of changes in SARS-CoV-2 spike protein in... Name: title, dtype: object [Arch Med Res, cord_uid ldf7uso2 2020 ldf7uso2 2020-03-18 Name: publish_time, dtype: object]Coronavirus spike proteins are glycosylated in nature where N-acetyl glucosamine (NAG) is the main component.
... Coronavirus spike proteins are glycosylated in nature where N-acetyl glucosamine (NAG) is the main component. Glycan shielding and possible epitope masking of an HCoV-NL63 has been observed which may be the barrier for proper immunogenic responses [8] . ...
Ref: Energetics based epitope screening in SARS CoV-2 (COVID 19) spike glycoprotein by Immuno-informatic analysis aiming to a suitable vaccine development [bioRxiv, 2020-04-05]The S protein is a heavily 143 glycosylated trimeric protein that mediates entry to host cells via fusion with ACE2.
... 2 The structure of the SARS-CoV-2 spike (S) protein is shown in Figure 3 . The S protein is a heavily 143 glycosylated trimeric protein that mediates entry to host cells via fusion with ACE2. Recently, Wrapp 144 and colleagues used Cryo-EM to determine the structure of the S protein ...
Ref: Evolutionary and structural analyses of SARS-CoV-2 D614G spike protein mutation now documented worldwide [bioRxiv, 2020-06-08]Pro330-Leu650 (SARS-CoV-2-SPL),
... to date. In this study, we analyzed the biological characteristics of the SARS-CoV-2 Spike protein, Pro330-Leu650 (SARS-CoV-2-SPL), using biostatistical methods. SARS-CoV-2-SPL possesses a receptor-binding region (RBD) and important B (Ser438-Gln506, Thr553-Glu583, Gly404-Aps427, ...
Ref: The biological characteristics of SARS-CoV-2 Spike protein Pro330-Leu650 [Vaccine, 2020-04-30]The 3D structure 205 of the spike protein RBD of SARS-CoV-2
... amino acid mutation to RBD may have significant impact on receptor binding and vaccine development. The 3D structure 205 of the spike protein RBD of SARS-CoV-2 (PDB: 6VW1) has recently been determined in complex with human ACE2 206 receptor (6). One ...
Ref: Analysis of the mutation dynamics of SARS-CoV-2 reveals the spread history and emergence of RBD mutant with lower ACE2 binding affinity [bioRxiv, 2020-04-11]The spike glycoprotein structure of SARS-CoV-2 resembles the spike protein of SARS-CoV with an RMSD of 3.8 Å.
... hemagglutinin-esterase gene. However, it comprises of 5 0 and 3 0 flanking untranslated regions (UTRs). The spike glycoprotein structure of SARS-CoV-2 resembles the spike protein of SARS-CoV with an RMSD of 3.8 Å. Like SARS-CoV, SARS-CoV-2 uses the ACE2 receptor for internalization and TMPRSS2 serine proteases for S ...
Ref: Morphology, Genome Organization, Replication, and Pathogenesis of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) [Coronavirus Disease 2019 (COVID-19), 2020-04-30]The viral spike protein binds the host receptor angiotensin-converting enzyme 2 (ACE2) via the receptor-binding domain (RBD),
... by the novel human coronavirus SARS-CoV-2, is currently a major threat to public health worldwide. The viral spike protein binds the host receptor angiotensin-converting enzyme 2 (ACE2) via the receptor-binding domain (RBD), and thus is believed to be a major target to block viral entry. Both SARS-CoV-2 ...
Ref: Key residues of the receptor binding motif in the spike protein of SARS-CoV-2 that interact with ACE2 and neutralizing antibodies [Cell Mol Immunol, 2020-05-15]The S protein is a homotrimer with each monomer comprised of 1281 amino acids.
... of ACE2 indicates a wide range of hosts (human and nonhuman) for SARS-CoV-2 (2) . The S protein is a homotrimer with each monomer comprised of 1281 amino acids. The monomers are expected to be highly glycosylated with 22 N-linked glycosylation sequons and 4 ...
Ref: Development and simulation of fully glycosylated molecular models of ACE2-Fc fusion proteins and their interaction with the SARS-CoV-2 spike protein binding domain [bioRxiv, 2020-05-06]The co-crystal structure of the spike protein of SARS-CoV complexed 69 to human-civet chimeric receptor ACE2
... bootstrap method with 1000 replicates. The resulting phylogenetic tree 65 was edited with iTOL [9]. The co-crystal structure of the spike protein of SARS-CoV complexed 69 to human-civet chimeric receptor ACE2 was solved at 3Å of resolution 70 (PDB code 3SCL). We used this structure as ...
Ref: Interaction of the spike protein RBD from SARS-CoV-2 with ACE2: similarity with SARS-CoV, hot-spot analysis and effect of the receptor polymorphism [bioRxiv, 2020-05-07]Spike glycoprotein is one of the major targets to be explored because of its role during the entry of coronaviruses into host cells.
... constituent genes and proteins need to be explored to understand the biology of the virus. Spike glycoprotein is one of the major targets to be explored because of its role during the entry of coronaviruses into host cells. We analyzed 320 whole-genome sequences and 320 spike protein sequences of SARS-CoV-2 using multiple sequence ...
Ref: Exploring the genomic and proteomic variations of SARS-CoV-2 spike glycoprotein: A computational biology approach [Infect Genet Evol, 2020]A structure-based Hamiltonian of trimeric spike protein for SAR-CoV2 is derived using the super-symmetric contact map.
... A structure-based Hamiltonian of trimeric spike protein for SAR-CoV2 is derived using the super-symmetric contact map. In the current structure-based model amino acids are represented by single beads at the location of the C-α atom (15, 25, 31, 32) . The coarse-grained structurebased model, a well-established ...
Ref: Dynamical asymmetry exposes 2019-nCoV prefusion spike [bioRxiv, 2020-04-21]The crystal structure of the C-terminal domain of the SARS-CoV-2 spike protein in complex with human ACE2
... The crystal structure of the C-terminal domain of the SARS-CoV-2 spike protein in complex with human ACE2 reveals insights into the mechanisms of binding of this virus and its differences from SARS. ...
Ref: Structural and Functional Basis of SARS-CoV-2 Entry by Using Human ACE2 [Cell, 2020-04-09]The spike protein is located on the virus envelope and promotes the attachment to the host cell and the fusion between the viral and cellular membrane.
... by the high-affinity interactions between the receptor-binding domain (RBD) of the spike (S) glycoprotein and the human-host Angiotensin converting enzyme 2 (ACE2) receptor [12] [13] [14] . The spike protein is located on the virus envelope and promotes the attachment to the host cell and the fusion between the viral and cellular membrane. [15, 16] . ...
Ref: In-Silico evidence for two receptors based strategy of SARS-CoV-2 [Cell, 2020-03-24]It is a large (approx. 180 kDa) glycoprotein that is present on the viral surface as a prominent trimer,
... the primary determinant of viral tropism and is responsible for receptor binding and membrane fusion. It is a large (approx. 180 kDa) glycoprotein that is present on the viral surface as a prominent trimer, and it is composed of two domains, S1 and S2 [23] . The S1 domain ...
Ref: Phylogenetic Analysis and Structural Modeling of SARS-CoV-2 Spike Protein Reveals an Evolutionary Distinct and Proteolytically Sensitive Activation Loop [J Mol Biol, 2020-04-19]The spike protein includes two regions, S1 and S2, where S1 is for host cell receptor binding and S2 is for membrane fusion.
... host cells and mediate host cell membrane and viral membrane fusion during infection [19] . The spike protein includes two regions, S1 and S2, where S1 is for host cell receptor binding and S2 is for membrane fusion. The S1 region also includes an N-terminal domain (NTD) and three C-terminal domains (CTD1, CTD2, ...
Ref: Molecular mechanism of evolution and human infection with the novel coronavirus (2019-nCoV) [bioRxiv, 2020-02-21]Spike (S) protein, envelope (E) protein, membrane (M) protein, and nucleocapsid (N) protein 10 .
... d). The SARS-CoV-2 belongs to the b genus. CoVs contain at least four structural proteins: Spike (S) protein, envelope (E) protein, membrane (M) protein, and nucleocapsid (N) protein 10 . Among them, Spike promotes host attachment and virusecell membrane fusion during virus infection. Therefore, Spike ...
Ref: Analysis of therapeutic targets for SARS-CoV-2 and discovery of potential drugs by computational methods [Acta Pharm Sin B, 2020-02-27]The SARS-CoV-2 spike protein binds to human Angiotensin-converting enzyme 2 (ACE2) mediating cell entry.
... The SARS-CoV-2 spike protein binds to human Angiotensin-converting enzyme 2 (ACE2) mediating cell entry. Subsequently, the ACE2 receptor binding domain has been the main target of most vaccine programs. Measures of selective pressure suggest that the spike protein is one of the viral proteins ...
Ref: COVID-3D: An online resource to explore the structural distribution of genetic variation in SARS-CoV-2 and its implication on therapeutic development [bioRxiv, 2020-05-30]20 spike glycoprotein structures,
... number of SARS-CoV-2 protein structures were released in the Protein Data Bank (PDB) [3] : 20 spike glycoprotein structures, over 100 main protease structures, and over 50 structures of other SARS-CoV-2 proteins, including nucleocapsid ...
Ref: CoV3D: A database of high resolution coronavirus protein structures [bioRxiv, 2020-06-01]SARS-CoV-2 and SARS-CoV share a common human receptor ACE2.
... SARS-CoV-2 and SARS-CoV share a common human receptor ACE2. Protein-protein interaction structure modeling indicates that spike-RBD of the two viruses also has similar overall binding conformation and binding free energy to ACE2. In vitro assays using recombinant ACE2 proteins ...
Ref: SARS-CoV-2 and SARS-CoV Spike-RBD Structure and Receptor Binding Comparison and Potential Implications on Neutralizing Antibody and Vaccine Development [bioRxiv, 2020-02-20]Spike (S) is a major protein in SARS-CoV-2 responsible for viral entry (4, 5) .
... increased transmission was recently correlated with high mutation frequency of the SARS-CoV-2 strain (3) . Spike (S) is a major protein in SARS-CoV-2 responsible for viral entry (4, 5) . Sprotein consists of N-terminal domain and C-terminal domain in the S1 region that binds to ...
Ref: Structural and Functional Implications of Spike Protein Mutational Landscape in SARS-CoV-2 [bioRxiv, 2020-06-13]receptor binding domain (RBD),
... the main conformational changes occurring in the SARS-CoV-2 spike protein, at the level of the receptor binding domain (RBD), along interactions with human cells angiotensin converting enzyme 2 (ACE2) receptor, that favour human cell ...
Ref: Protein structure analysis of the interactions between SARS-CoV-2 spike protein and the human ACE2 receptor: from conformational changes to novel neutralizing antibodies [bioRxiv, 2020-04-18]The spike protein is a trimeric class I fusion protein and consists of two subunits, namely S1 and S2.
... The pathogenic SARS-CoV-2 enters human target cells via its viral transmembrane spike (S) glycoprotein. The spike protein is a trimeric class I fusion protein and consists of two subunits, namely S1 and S2. The S1 subunit facilitates the attachment of the virus, and subsequently the S2 subunit allows for ...
Ref: Deducing the N- and O- glycosylation profile of the spike protein of novel coronavirus SARS-CoV-2 [bioRxiv, 2020-04-06]The receptor-binding domain (RBD) of the SARS-CoV-2 spike protein
... The receptor-binding domain (RBD) of the SARS-CoV-2 spike protein plays a crucial role in binding the human cell receptor ACE2 that is required for viral entry. Many studies have been conducted to target the structures of RBD-ACE2 binding and ...
Ref: The Distal Polybasic Cleavage Sites of SARS-CoV-2 Spike Protein Enhance Spike Protein-ACE2 Binding [bioRxiv, 2020-06-10]SARS-CoV-2 Mpro is made up of 306 amino acid residues.
... protease, and thereby maintain the shape of the substrate-binding pocket of S1 protein [59] . SARS-CoV-2 Mpro is made up of 306 amino acid residues. Crystal structure analysis suggests that Thr25, Thr26, Leu27, His41, Ser46, Met49, Tyr54, Phe140, Leu141, Asn142, ...
Ref: Corona virus versus existence of human on the earth: A computational and biophysical approach [Int J Biol Macromol, 2020-06-05]The simulated structures indicated that ACE2 proteins from Bovidae and Cricetidae were able to associate with SARS-CoV-2 RBD.
... and Cetacea maintained the majority of key residues in ACE2 for associating with SARS-CoV-2 RBD. The simulated structures indicated that ACE2 proteins from Bovidae and Cricetidae were able to associate with SARS-CoV-2 RBD. We found that nearly half of the key residues in turtle, snake, and bird were ...
Ref: SARS-CoV-2 spike protein favors ACE2 from Bovidae and Cricetidae [J. med. virol, 2020]Spike (S) protein, one of the structural proteins of this virus plays key role in receptor (ACE2) binding and thus virus entry.
... of SARS-CoV-2 late in 2019 (also named as COVID-19 or novel coronavirus 2019 or nCoV2019). Spike (S) protein, one of the structural proteins of this virus plays key role in receptor (ACE2) binding and thus virus entry. Thus, this protein has attracted scientists for detailed study and therapeutic targeting. As the nCoV2019 ...
Ref: A virus that has gone viral: amino acid mutation in S protein of Indian isolate of Coronavirus COVID-19 might impact receptor binding, and thus, infectivity [Biosci Rep, 2020-05-15]Each subunit of the SARS-CoV-2 trimeric spike protein has one polybasic cleavage site (R 682RAR685).
Each subunit of the SARS-CoV-2 trimeric spike protein has one polybasic cleavage site (R 682RAR685). 1
Ref: The Distal Polybasic Cleavage Sites of SARS-CoV-2 Spike Protein Enhance Spike Protein-ACE2 Binding [bioRxiv, 2020-06-10]These viruses make use of a large envelope protein called spike (S) to engage host cell receptors and catalyze membrane fusion.
... (SARS-CoV-1) and COVID-19 coronavirus (SARS-CoV-2) have all emerged into the human population with devastating consequences. These viruses make use of a large envelope protein called spike (S) to engage host cell receptors and catalyze membrane fusion. Because of the vital role that these S proteins play, they represent a vulnerable target ...
Ref: Structural Basis for Potent Neutralization of Betacoronaviruses by Single-domain Camelid Antibodies [bioRxiv, 2020-03-28]Spike protein is the outermost structural protein of the virus which interacts with the human receptor ACE2 and enters the respiratory system.
... temperature sensitivity of the virus. However, we still lack molecular level understanding of the same. Spike protein is the outermost structural protein of the virus which interacts with the human receptor ACE2 and enters the respiratory system. It is also one of largest proteins which have primary exposure to external environmental conditions. ...
Ref: Investigation of the effect of temperature on the structure of SARS-Cov-2 Spike Protein by Molecular Dynamics Simulations [bioRxiv, 2020-06-11]X-ray crystal structure of known antibody-antigen complex from 202 SARS and MERS (Fig. 3) .
... peer-reviewed) is the . https://doi.org/10.1101/2020.02.22.951178 doi: bioRxiv preprint structure of SARS-CoV-2 RBD protein at the X-ray crystal structure of known antibody-antigen complex from 202 SARS and MERS (Fig. 3) . The structures of five antibodies including m396, 80R, F26G19, S230, and CR3022 203 developing to ...
Ref: Spike protein binding prediction with neutralizing antibodies of SARS-CoV-2 [bioRxiv, 2020-02-27]The knowledge of the 3D structures of SARS-CoV-2 proteins can facilitate the development of therapeutic and diagnostic molecules.
... major threat to human health, and its diffusion around the world is causing dramatic consequences. The knowledge of the 3D structures of SARS-CoV-2 proteins can facilitate the development of therapeutic and diagnostic molecules. Specifically, comparative analyses of the structures of SARS-CoV-2 proteins and homologous proteins from previously characterized ...
Ref: Differential Antibody Recognition by Novel SARS-CoV-2 and SARS-CoV Spike Protein Receptor Binding Domains: Mechanistic Insights and Implications for the Design of Diagnostics and Therapeutics [bioRxiv, 2020-03-15]The viral spike (S) glycoprotein binds the receptor (angiotensin-converting enzyme 2) ACE2
... of the novel SARS-coronavirus 2 (SARS-CoV-2) and its international spread pose a global health emergency. The viral spike (S) glycoprotein binds the receptor (angiotensin-converting enzyme 2) ACE2 and promotes SARS-CoV-2 entry into host cells. The trimeric S protein binds the receptor using ...
Ref: Structural basis of SARS-CoV-2 spike protein induced by ACE2 [bioRxiv, 2020-05-24]The glycoprotein spike (S) on the surface of SARS-CoV-2 is a determinant for viral invasion and host immune response.
... The glycoprotein spike (S) on the surface of SARS-CoV-2 is a determinant for viral invasion and host immune response. Herein, we characterized the site-specific N-glycosylation of S protein at the level of intact glycopeptides. All 22 potential N-glycosites were identified in the S-protein protomer and were found to be ...
Ref: Site-specific N-glycosylation Characterization of Recombinant SARS-CoV-2 Spike Proteins [bioRxiv, 2020-04-19]S-ectodomain (S1+S2) (aa 16-1213),
... response induced by spike protein-based vaccines, we immunized rabbits with various SARS-CoV-2 spike protein antigens: S-ectodomain (S1+S2) (aa 16-1213), which lacks the cytoplasmic and transmembrane domains (CT-TM), the S1 domain (aa 16-685), the receptor-binding ...
Ref: Antibody repertoire induced by SARS-CoV-2 spike protein immunogens [bioRxiv, 2020-05-13]The attachment of the virus with the cell-surface receptor and a cofactor is the first step for the infection.
... number of cases and deaths, but our understanding about the pathogen SARS-CoV-2 remains largely unclear. The attachment of the virus with the cell-surface receptor and a cofactor is the first step for the infection. Here, bioinformatics approaches combining human-virus protein interaction prediction and protein docking based on crystal structures ...
Ref: cord_uid 1xf2sxtv The MERS-CoV Receptor DPP4 as a Candidate Bind... 1xf2sxtv The MERS-CoV receptor DPP4 as a candidate bind... Name: title, dtype: object [bioRxiv, cord_uid 1xf2sxtv 2020-05-13 1xf2sxtv 2020-05-13 Name: publish_time, dtype: object]Neutralizing antibody responses to coronaviruses focus on the trimeric spike, with most against the receptor-binding domain (RBD).
... Neutralizing antibody responses to coronaviruses focus on the trimeric spike, with most against the receptor-binding domain (RBD). Here we characterized polyclonal IgGs and Fabs from COVID-19 convalescent individuals for recognition of coronavirus spikes. Plasma IgGs differed in their degree of focus on RBD epitopes, recognition of SARS-CoV, ...
Ref: Structures of human antibodies bound to SARS-CoV-2 spike reveal common epitopes and recurrent features of antibodies [bioRxiv, 2020-05-29]The 3D structure of spike protein of SARS-CoV-2
The 3D structure of spike protein of SARS-CoV-2 (NCBI Reference Sequence: YP_009724390.1) was generated based on homologous modeling using Modeller(10) incorporated within the UCSF Chimera (11) and SWISS-MODEL (12) .
Ref: The potential SARS-CoV-2 entry inhibitor [bioRxiv, 2020-03-26]receptor-binding domain (RBD) of the spike protein of SARS-CoV-2 bound to the cell receptor ACE2.
... initial step of infection at an atomic level, we determined the crystal structure of the receptor-binding domain (RBD) of the spike protein of SARS-CoV-2 bound to the cell receptor ACE2. The overall ACE2-binding mode of the SARS-CoV-2 RBD is nearly identical to that of the ...
Ref: Structure of the SARS-CoV-2 spike receptor-binding domain bound to the ACE2 receptor [Nature, 2020]The spike protein (S protein) of SARS-CoV-2 consists of S1 and S2 subunits on the viral membrane,
... The detailed mechanism underlying SARS-CoV-2 entering the host cell is unclear. The spike protein (S protein) of SARS-CoV-2 consists of S1 and S2 subunits on the viral membrane, which is similar to SARS-CoV. The S1 subunit contains the receptorbinding domain (RBD) for binding the cellular receptor. Furin, ...
Ref: In Silico Design of Antiviral Peptides Targeting the Spike Protein of SARS-CoV-2 [Peptides, 2020-05-05]Here, we investigated the structural properties of several ACE2 orthologs bound to the SARS-CoV-2 spike protein.
... whether human-to-animal transmission is possible and if so, which animal species are most at risk. Here, we investigated the structural properties of several ACE2 orthologs bound to the SARS-CoV-2 spike protein. We found that species known not to be susceptible to SARS-CoV-2 infection have non-conservative mutations ...
Ref: Insights on cross-species transmission of SARS-CoV-2 from structural modeling [bioRxiv, 2020-06-05]One of the structural proteins, the spike surface glycoprotein ('spike glycoprotein'), is one of the primary therapeutic targets.
... so-called structural proteins such as spike glycoproteins, an envelope protein, membrane proteins, and the nucleocapsid. One of the structural proteins, the spike surface glycoprotein ("spike glycoprotein"), is one of the primary therapeutic targets. This protein plays an important role in binding to receptors on the host cell, fusion ...
Ref: SARS‐CoV‐2: Structural diversity, phylogeny, and potential animal host identification of spike glycoprotein [J Med Virol, 2020-05-17]The heavily glycosylated trimeric surface Spike protein mediates viral entry into the host cell.
... B betacoronavirus and sharing 88% sequence identity with bat coronaviruses (Lu et al., 2020a) . The heavily glycosylated trimeric surface Spike protein mediates viral entry into the host cell. It is a large type I transmembrane glycoprotein (the ectodomain alone comprises over 1200 residues) ...
Ref: Neutralization of SARS-CoV-2 by destruction of the prefusion Spike [bioRxiv, 2020-05-06]The spike protein is located on the virus envelope and promotes the attachment to the host cell and the fusion between the virus and the cellular membrane.
... mediated by the high-affinity interactions between the receptor-binding domain (RBD) of the spike (S) glycoprotein and the human-host Angiotensin-converting enzyme 2 (ACE2) receptor [12] [13] [14] . The spike protein is located on the virus envelope and promotes the attachment to the host cell and the fusion between the virus and the cellular membrane. [15, 16] . ...
Ref: In-Silico evidence for two receptors based strategy of SARS-CoV-2 [bioRxiv, 2020-04-06]a large trimeric class I fusion protein that is metastable and difficult to produce recombinantly in large quantities.
... this public health emergency. A key target of these efforts is the spike (S) protein, a large trimeric class I fusion protein that is metastable and difficult to produce recombinantly in large quantities. Here, we designed and expressed over 100 structure-guided spike variants based upon a previously determined ...
Ref: Structure-based Design of Prefusion-stabilized SARS-CoV-2 Spikes [bioRxiv, 2020-05-30]The spike protein of SARS-CoV-2 is comprised of two subunits;
... the host. 1 SARS-CoV is closely related to SARS-CoV-2 and employs the same receptor. 2 The spike protein of SARS-CoV-2 is comprised of two subunits; S1 is responsible for binding to the host receptor, whereas S2 promotes membrane fusion. The ...
Ref: SARS-CoV-2 spike protein binds heparan sulfate in a length- and sequence-dependent manner [bioRxiv, 2020-05-10]The SARS-CoV-2 spike protein (S) is the main molecule present at the surface of the virion.
... The SARS-CoV-2 spike protein (S) is the main molecule present at the surface of the virion. This large glycoprotein assembles in trimers that form a crown-like structure on the envelope which gives this virus family its name (corona = crown) (Fig. 1) . The spike protein ...
Ref: A potential role for integrins in host cell entry by SARS-CoV-2 [Antiviral Res, 2020-03-01]Spike S is assembled as a trimer and contains around 1,300 amino acids within each unit 8, 9 .
... activates the infection in human respiratory epithelial cells in SARS-CoV, MERS-CoV and SARS-CoV-2 7 . Spike S is assembled as a trimer and contains around 1,300 amino acids within each unit 8, 9 . The receptor binding domain (RBD) of Spike S, which contains around 300 amino acids, mediates ...
Ref: Structural analysis of SARS-CoV-2 and prediction of the human interactome [Antiviral Res, 2020-03-30]On mature viruses, the spike protein is present as a trimer,
... A virus surface-anchored spike protein mediates coronavirus entry ( Fig. 1 B and C) . On mature viruses, the spike protein is present as a trimer, with three receptor-binding S1 heads sitting on top of a trimeric membrane fusion S2 stalk ...
Ref: Cell entry mechanisms of SARS-CoV-2 [Proc Natl Acad Sci U S A, 2020-05-26]receptor binding domain (RBD), along interactions with human cells angiotensin converting enzyme 2 (ACE2) receptor,
... the main conformational changes occurring in the SARS-CoV-2 spike protein, at the level of the receptor binding domain (RBD), along interactions with human cells angiotensin converting enzyme 2 (ACE2) receptor, that favour human cell invasion. Furthermore, our analysis provides i) an ideal pipeline to identify ...
Ref: Protein structure analysis of the interactions between SARS-CoV-2 spike protein and the human ACE2 receptor: from conformational changes to novel neutralizing antibodies [bioRxiv, 2020-04-18]comprises Cys336-Gly526 residues according to the sequence homology analysis with SARS-CoV spike RBD.
... The spike RBD of SARS-CoV-2 (GenBank: MN908947 2) comprises Cys336-Gly526 residues according to the sequence homology analysis with SARS-CoV spike RBD. The predicted three-dimensional structure model of these residues was obtained with the SWISS-MODEL server 11. This predicted SARS-CoV-2 RBD model was subsequently ...
Ref: Computational simulations reveal the binding dynamics between human ACE2 and the receptor binding domain of SARS-CoV-2 spike protein [bioRxiv, 2020-03-27]the spike glycoprotein (S-protein), the envelope protein, membrane protein, and nucleocapsid.
... the largest known RNA virus genome. The coronavirus spherical virion consists of four structural proteins: the spike glycoprotein (S-protein), the envelope protein, membrane protein, and nucleocapsid. The transmembrane trimeric S-protein plays a critical role in virus entry into host cells (Gallagher ...
Ref: SARS‐CoV‐2, an evolutionary perspective of interaction with human ACE2 reveals undiscovered amino acids necessary for complex stability [Evol Appl, 2020-05-07]at the level of the receptor binding domain (RBD),
... to gain new insights about the main conformational changes occurring in the SARS-CoV-2 spike protein, at the level of the receptor binding domain (RBD), along interactions with human cells angiotensin converting enzyme 2 (ACE2) receptor, that favour human cell ...
Ref: Protein structure analysis of the interactions between SARS-CoV-2 spike protein and the human ACE2 receptor: from conformational changes to novel neutralizing antibodies [bioRxiv, 2020-04-18]A CryoEM-derived structure of the SARS-CoV-2 spike protein became available on Feb. 19, 2020
... A CryoEM-derived structure of the SARS-CoV-2 spike protein became available on Feb. 19, 2020 and was publicly released Feb 26, 2020 [18] (https://www.rcsb.org/structure/6vsb). X-ray crystal structures (of the receptor-binding domain) became available starting March 4, 2020 (http://www.rcsb.org/structure/6VW1 , to be published). Comparison with our ...
Ref: Rapid in silico design of antibodies targeting SARS-CoV-2 using machine learning and supercomputing [bioRxiv, 2020-04-10]glycosylated spike proteins recognize the human angiotensin converting enzyme-2 (ACE-2) receptor.
... more rapidly and readily than SARS CoV. Both, SARS CoV and SARS CoV-2 via their glycosylated spike proteins recognize the human angiotensin converting enzyme-2 (ACE-2) receptor. We generated multiple sequence alignments and phylogenetic trees for representative spike proteins of SARS CoV ...
Ref: Evolutionary relationships and sequence-structure determinants in human SARS coronavirus-2 spike proteins for host receptor recognition. [Proteins, 2020-06-16]The complex structures were superimposed to the RBD 125 structure of SARS-CoV-2 which were built by homology modeling.
... ). The complex structure of RBD and ten 124 neutralizing antibodies was retrieved from PDB. The complex structures were superimposed to the RBD 125 structure of SARS-CoV-2 which were built by homology modeling. The procedures were performed that the 126 RBD structures of SARS-CoV2 and SARS-CoV were aligned ...
Ref: Spike protein binding prediction with neutralizing antibodies of SARS-CoV-2 [bioRxiv, 2020-02-27]The SARS-CoV-2 surface glycoprotein termed spike protein is composed of two subunits (S1 and S2);
... The SARS-CoV-2 surface glycoprotein termed spike protein is composed of two subunits (S1 and S2); with the S1 subunit including the receptor binding domain (RBD) (Figure 1a) . To generate SARS-CoV-2 spike proteins, the ectodomain of spike was expressed in 293T/17 cells using a previously ...
Ref: Identification of IgG antibody response to SARS-CoV-2 spike protein and its receptor binding domain does not predict rapid recovery from COVID-19 [bioRxiv, 2020-05-06]receptor-binding domain (RBD) bound to the cell receptor ACE2 at 2.45 Å resolution.
... of infection at an atomic level, we determined the crystal structure of the SARS-CoV-2 spike receptor-binding domain (RBD) bound to the cell receptor ACE2 at 2.45 Å resolution. The overall ACE2-binding mode of the SARS-CoV-2 RBD is nearly identical to that of the ...
Ref: Structure of the SARS-CoV-2 spike receptor-binding domain bound to the ACE2 receptor. [Nature, 2020-03-30]the structure of the S1-CTD of the SARS-CoV-2 S protein,
... to what have been observed in the structures of the SARS-CoV and MERS-CoV S proteins, the structure of the S1-CTD of the SARS-CoV-2 S protein, which is located at the apex of the S protein trimer, is very flexible and ...
Ref: Binding of the SARS-CoV-2 Spike Protein to Glycans [bioRxiv, 2020-05-17]The simulated structures indicated that ACE2 proteins from Bovidae and Cricetidae were able to associate with SARS‐CoV‐2 RBD.
... and Cetacea maintained the majority of key residues in ACE2 for associating with SARS‐CoV‐2 RBD. The simulated structures indicated that ACE2 proteins from Bovidae and Cricetidae were able to associate with SARS‐CoV‐2 RBD. We found that nearly half of the key residues in turtle, snake, and bird were ...
Ref: SARS‐CoV‐2 spike protein favors ACE2 from Bovidae and Cricetidae [J Med Virol, 2020-04-10]We analyzed 320 whole-genome sequences and 320 spike protein sequences of SARS-CoV-2 using multiple sequence alignment.
... to be explored because of its role during the entry of coronaviruses into host cells. We analyzed 320 whole-genome sequences and 320 spike protein sequences of SARS-CoV-2 using multiple sequence alignment. In this study, 483 unique variations have been identified among the genomes of SARS-CoV-2 including ...
Ref: Exploring the genomic and proteomic variations of SARS-CoV-2 spike glycoprotein: A computational biology approach [Infect Genet Evol, 2020]The transmembrane spike (S) glycoprotein of SARS-CoV-2 directly binds to the host angiotensin-converting enzyme 2 (ACE2)
... global threat for which there is an urgent need to search for an effective therapy. The transmembrane spike (S) glycoprotein of SARS-CoV-2 directly binds to the host angiotensin-converting enzyme 2 (ACE2) and mediates viral entrance, which is therefore considered as a promising drug target. Considering that ...
Ref: Eltrombopag is a potential target for drug intervention in SARS-CoV-2 spike protein [Infect Genet Evol, 2020-06-12]The viral spike glycoprotein (S) utilizes angiotensin-converting enzyme 2 (ACE2) as a host protein receptor
... acute respiratory syndrome coronavirus (SARS-CoV) emerged in 2002 as a highly transmissible pathogenic human betacoronavirus. The viral spike glycoprotein (S) utilizes angiotensin-converting enzyme 2 (ACE2) as a host protein receptor and mediates fusion of the viral and host membranes, making S essential to viral entry ...
Ref: Stabilized coronavirus spikes are resistant to conformational changes induced by receptor recognition or proteolysis [Sci Rep, 2018-10-24]The modelled structure comprised of both extracellular and helical domains with the amino acids ranging from 21 to 768.
... a potential template with an amino acid identity of 100% and query coverage of 99%. The modelled structure comprised of both extracellular and helical domains with the amino acids ranging from 21 to 768. Structure validation using the Ramachandran plot has confirmed 91.4% residues in the favoured region; 8.2% ...
Ref: A computational insight of the improved nicotine binding with ACE2-SARS-CoV-2 complex with its clinical impact [Sci Rep, 2020-04-30]S-protein of SARS-CoV-2 has conserved tertiary folds that are involved in interaction with human receptors,
... (S-protein) of coronaviruses interacts with specific membrane receptors for their entry into the human cells. S-protein of SARS-CoV-2 has conserved tertiary folds that are involved in interaction with human receptors, but has limited sequence similarity with previously known coronaviruses (such as SARS-CoV, MERS-CoV) . Multiple ...
Ref: Assessment of risk conferred by coding and regulatory variations of TMPRSS2 and CD26 in susceptibility to SARS-CoV-2 infection in human [J Genet, 2020-06-09]2.7 Å crystal structure of the N-terminal RNA binding domain of SARS-CoV-2 nucleocapsid protein.
... information of SARS-CoV-2 nucleocapsid protein is yet to be clear. Herein, we have determined the 2.7 Å crystal structure of the N-terminal RNA binding domain of SARS-CoV-2 nucleocapsid protein. Although overall structure is similar with other reported coronavirus nucleocapsid protein N-terminal domain, the surface ...
Ref: Crystal structure of SARS-CoV-2 nucleocapsid protein RNA binding domain reveals potential unique drug targeting sites [bioRxiv, 2020-03-07]The spike protein protrudes from the envelope of the virion and plays a pivotal role in the receptor host selectivity and cellu-lar attachment.
... The viral particles contain four main structural proteins: the spike, membrane, envelope protein, and nucleocapsid. The spike protein protrudes from the envelope of the virion and plays a pivotal role in the receptor host selectivity and cellu-lar attachment. Strong scientific evidence showed that SARS and SARS-CoV-2 spike proteins interact with angiotensin-converting enzyme 2 ...
Ref: cord_uid ldf7uso2 Role of changes in SARS-CoV-2 spike protein in... ldf7uso2 Role of changes in SARS-CoV-2 spike protein in... Name: title, dtype: object [bioRxiv, cord_uid ldf7uso2 2020 ldf7uso2 2020-03-18 Name: publish_time, dtype: object]First, virions land on the epithelial surface in the airway by binding to HS through their SGPs (Fig 6A) .
... a model on how GAGs may facilitate host cell entry of SARS-CoV-2 (Fig 6) . First, virions land on the epithelial surface in the airway by binding to HS through their SGPs (Fig 6A) . Host cell surface proteoglycans utilize their long HS chains to securely wrap around the trimeric ...
Ref: Glycosaminoglycan binding motif at S1/S2 proteolytic cleavage site on spike glycoprotein may facilitate novel coronavirus (SARS-CoV-2) host cell entry [bioRxiv, 2020-04-15]The S-protein forms trimers at the protrusions of the virus and comprises two functional subunits : S1 and S2.
... a potential strategy to tackle the viral entry into human cells (8) (9) (10) . The S-protein forms trimers at the protrusions of the virus and comprises two functional subunits : S1 and S2. In the cascade of the viral entry, The S1 unit of the spike (S) protein ...
Ref: The inhibitory effect of a Corona virus spike protein fragment with ACE2 [bioRxiv, 2020-06-03]We review the viral structure, size, rigidity, lipophilicity, isoelectric point, buoyant density and centrifugation conditions,
... properties of the severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) and its proteins are discussed. We review the viral structure, size, rigidity, lipophilicity, isoelectric point, buoyant density and centrifugation conditions, stability against pH, temperature, UV light, gamma radiation, and susceptibility to various chemical agents including ...
Ref: Physicochemical properties of SARS‐CoV‐2 for drug targeting, virus inactivation and attenuation, vaccine formulation and quality control [Electrophoresis, 2020-06-08]Here, we present the 2.7 Å crystal structure of SARS-CoV-2 N protein N-terminal domain,
... 11 . However, the molecular basis of SARS-CoV-2 N protein is yet to be elucidated. Here, we present the 2.7 Å crystal structure of SARS-CoV-2 N protein N-terminal domain, revealing the specific surface charge distributions which may facilitate drug discovery specifically to SARS-CoV-2 N ...
Ref: Crystal structure of SARS-CoV-2 nucleocapsid protein RNA binding domain reveals potential unique drug targeting sites [Acta Pharm Sin B, 2020-04-20]The receptor-binding domain
... The receptor-binding domain (RBD) of the SARS-CoV-2 spike protein plays a crucial role in binding the human cell receptor ACE2 that is required for viral entry. Many studies have been conducted to target ...
Ref: The Distal Polybasic Cleavage Sites of SARS-CoV-2 Spike Protein Enhance Spike Protein-ACE2 Binding [bioRxiv, 2020-06-10]The CoV spike protein binds to a host cell membrane through a receptor-mediated interaction which allows entrance to the host cell.
... The CoV spike protein binds to a host cell membrane through a receptor-mediated interaction which allows entrance to the host cell. It has been computationally determined that the SARS-CoV-2 has similar mechanism to that of the SARS virus and the receptor to which it has the highest affinity is ACE2 (angiotensin-converting ...
Ref: A search for medications to treat COVID-19 via in silico molecular docking models of the SARS-CoV-2 spike glycoprotein and 3CL protease [Travel Med Infect Dis, 2020-04-12]The domain-specific residuerange for the full-length, trimeric SARS-CoV-2 S is given in Fig. 2A .
... template structure (pdb: 6vsb) (7, 16) . This also helped to build the missing loops. The domain-specific residuerange for the full-length, trimeric SARS-CoV-2 S is given in Fig. 2A . The S1 head coordination of the trimeric spike is programmed by developing a super-symmetric topology-based ...
Ref: Dynamical asymmetry exposes 2019-nCoV prefusion spike [bioRxiv, 2020-04-21]The SARS-CoV-2 spike protein is a homotrimer composed of three monomers
... The SARS-CoV-2 spike protein is a homotrimer composed of three monomers (chains A, B, and C; Figure 1a ). Each monomeric protein contains an N-terminal ACE2 binding domain (receptor binding domain; RBD), a central helix/heptad repeat, and a C-terminal region that ...
Ref: Highly Conserved Homotrimer Cavity Formed by the SARS-CoV-2 Spike Glycoprotein: A Novel Binding Site [J Clin Med, 2020-05-14]A virus surface spike protein mediates SARS-CoV-2 entry into cells.
... enters human cells is a high priority for deciphering its mystery and curbing its spread. A virus surface spike protein mediates SARS-CoV-2 entry into cells. To fulfill its function, SARS-CoV-2 spike binds to its receptor human ACE2 (hACE2) through its ...
Ref: Cell entry mechanisms of SARS-CoV-2 [Proc Natl Acad Sci U S A, 2020]ACE2 is available in complete by S1 protein of SARS-COV-2 for protein-protein interaction.
... sequence of Homo sapiens was obtained from the Uniprot database (Accession Number: Q9BYF1) (13) . ACE2 is available in complete by S1 protein of SARS-COV-2 for protein-protein interaction. Search for a potential template for ACE2 from Protein Data Bank (14) listed PDB ID: ...
Ref: A computational insight of the improved nicotine binding with ACE2-SARS-CoV-2 complex with its clinical impact [Proc Natl Acad Sci U S A, 2020-04-30]20 spike glycoprotein structures, over 100 main protease structures,
... number of SARS-CoV-2 protein structures were released in the Protein Data Bank (PDB) [3] : 20 spike glycoprotein structures, over 100 main protease structures, and over 50 structures of other SARS-CoV-2 proteins, including nucleocapsid and RNA dependent RNA polymerase, ...
Ref: CoV3D: A database of high resolution coronavirus protein structures [bioRxiv, 2020-06-01]S protein, which is a type I glycoprotein, protrudes from the surface of the virus and can contact the host cell earlier.
... proteins, namely spike protein (S), membrane protein (M), envelope protein (E), and nucleocapsid protein (N) [6] . S protein, which is a type I glycoprotein, protrudes from the surface of the virus and can contact the host cell earlier. S protein has attracted great attention because of its function in receptor binding. ...
Ref: Spike protein recognition of mammalian ACE2 predicts the host range and an optimized ACE2 for SARS-CoV-2 infection [Biochem Biophys Res Commun, 2020-03-19]The first structures of the SARS-CoV-2 trimeric spike glycoproteins
... of immense importance for understanding viral assembly and to aid rational vaccine and therapeutic design. The first structures of the SARS-CoV-2 trimeric spike glycoproteins (the major target of SARS-CoV-2 vaccines and antibody therapeutics) were reported in February and early ...
Ref: CoV3D: A database of high resolution coronavirus protein structures [bioRxiv, 2020-06-01]The spike protein (S) of this virus enables binding to the human receptor ACE2,
... demands urgent solutions for detection and therapy to prevent escalating health, social and economic impacts. The spike protein (S) of this virus enables binding to the human receptor ACE2, and hence presents a prime target for vaccines preventing viral entry into host cells1. The ...
Ref: Functional pangenome analysis suggests inhibition of the protein E as a readily available therapy for COVID-2019 [bioRxiv, 2020-02-21]the SARS-CoV-2 has proteins, such as the Spike (S) glycoprotein,
... each age range. 19 Despite the fact that the virus" molecular mechanism is partially unknown, the SARS-CoV-2 has proteins, such as the Spike (S) glycoprotein, that densely decorates the viral external J o u r n a l P r ...
Ref: On the interactions of the receptor-binding domain of SARS-CoV-1 and SARS-CoV-2 spike proteins with monoclonal antibodies and the receptor ACE2 [Virus Res, 2020-05-15]The SARS-Cov-2 Spike protein structure consists of three chains or protomers (A, B, and C chains)
... The SARS-Cov-2 Spike protein structure consists of three chains or protomers (A, B, and C chains) of which the chain A is given in the so-called "Up" state of its RBD (6vsb.pdb), and chains B and C are in their "Down" state. We energetically mapped the ...
Ref: Static All-Atom Energetic Mappings of the SARS-Cov-2 Spike Protein with Potential Latch Identification of the Down State Protomer [bioRxiv, 2020-05-12]The spike protein bears a large ectodomain that is composed of a subunit S1
... nonetheless, significant differences were Figure 1 : A schematic view of the coronavirus spike protein. The spike protein bears a large ectodomain that is composed of a subunit S1 which has at the top the Receptor Binding Domain (RBD) and the subunit S2. It ...
Ref: Elucidating the differences in the molecular mechanism of receptor binding between 2019-nCoV and the SARS-CoV viruses using computational tools [bioRxiv, 2020-04-21]Cys336-Gly526 residues according to the sequence homology analysis with SARS-CoV spike RBD.
... The spike RBD of SARS-CoV-2 (GenBank: MN908947 2) comprises Cys336-Gly526 residues according to the sequence homology analysis with SARS-CoV spike RBD. The predicted three-dimensional structure model of these residues was obtained with the SWISS-MODEL server 11. This predicted SARS-CoV-2 RBD model was ...
Ref: Computational simulations reveal the binding dynamics between human ACE2 and the receptor binding domain of SARS-CoV-2 spike protein [bioRxiv, 2020-03-27]at the level of the receptor binding domain (RBD), along interactions with human cells angiotensin converting enzyme 2 (ACE2) receptor,
... to gain new insights about the main conformational changes occurring in the SARS-CoV-2 spike protein, at the level of the receptor binding domain (RBD), along interactions with human cells angiotensin converting enzyme 2 (ACE2) receptor, that favour human cell invasion. Furthermore, our analysis provides i) an ideal pipeline to identify ...
Ref: Protein structure analysis of the interactions between SARS-CoV-2 spike protein and the human ACE2 receptor: from conformational changes to novel neutralizing antibodies [bioRxiv, 2020-04-18]The viral proteins, often highly glycosylated, can be recognized by different carbohydrate-binding proteins from the host,
... shown for many viruses such as HIV-1, Influenza viruses, Ebolavirus and SARS-CoV [4, 5] . The viral proteins, often highly glycosylated, can be recognized by different carbohydrate-binding proteins from the host, triggering simultaneously immune-responses that can facilitate or inhibit viral entry or dissemination [5] . In ...
Ref: Novel ACE2-Independent Carbohydrate-Binding of SARS-CoV-2 Spike Protein to Host Lectins and Lung Microbiota [bioRxiv, 2020-05-14]